Journal article
MicroRNA-200c Attenuates Periodontitis by Modulating Proinflammatory and Osteoclastogenic Mediators
Stem cells and development, Vol.28(15), pp.126-1036
08/01/2019
DOI: 10.1089/scd.2019.0027
PMID: 31017046
Abstract
This study tested whether microRNA
(miR)-200c
can attenuate the inflammation and alveolar bone resorption in periodontitis by using an in vitro and a rat model. Polyethylenimine (PEI) was used to facilitate the transfection of plasmid DNA encoding
miR-200c
into primary human gingival fibroblasts (HGFs) and gingival tissues of rats. We first analyzed how proinflammatory and osteoclastogenic mediators in HGFs with overexpression of
miR-200c
responded to
Porphyromonas gingivalis
lipopolysaccharide (LPS-PG) challenge in vitro. We observed that overexpression of
miR-200c
significantly reduced interleukin (IL)-6 and 8 and repressed interferon-related developmental regulator-1
(IFRD1)
in HGFs.
miR-200c
also downregulated
p65
and
p50
. In a rat model of periodontitis induced by an LPS injection at the gingival sulcus of the second maxillary molar (M2), we analyzed how the mediators in rat gingiva and alveolar bone resorption responded to
miR-200c
treatment by a local injection of PEI-plasmid
miR-200
nanoplexes. We observed that the local injection of
miR-200c
significantly upregulated
miR-200c
expression in gingiva and reduced
IL-6, IL-8, IFRD1
, and the ratio of receptor activator of nuclear factor kappa-B ligand/osteoprotegerin. Using micro-computed tomography analysis and histomorphometry, we further confirmed that local treatment with
miR-200c
effectively protected alveolar bone resorption in the rat model of periodontitis by reducing the distance between the cemento-enamel junction and the alveolar bone crest and the inter-radicular space in the upper maxilla at M2. These findings imply that
miR-200c
may serve as a unique means to prevent periodontitis and associated bone loss.
Details
- Title: Subtitle
- MicroRNA-200c Attenuates Periodontitis by Modulating Proinflammatory and Osteoclastogenic Mediators
- Creators
- Adil Akkouch - 1Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IowaMin Zhu - 1Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IowaMiguel Romero-Bustillos - 2Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, IowaSteven Eliason - 4Department of Anatomy and Cell Biology, Carver College of Medicine, The University of Iowa, Iowa City, IowaFang Qian - 1Iowa Institute for Oral Health Research, College of Dentistry, The University of Iowa, Iowa City, IowaAliasger K Salem - 5Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IowaBrad A Amendt - 4Department of Anatomy and Cell Biology, Carver College of Medicine, The University of Iowa, Iowa City, IowaLiu Hong - 3Center for Craniofacial Anomalies Research, Carver College of Medicine, The University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Stem cells and development, Vol.28(15), pp.126-1036
- DOI
- 10.1089/scd.2019.0027
- PMID
- 31017046
- NLM abbreviation
- Stem Cells Dev
- ISSN
- 1547-3287
- eISSN
- 1557-8534
- Publisher
- Mary Ann Liebert, Inc., publishers
- Language
- English
- Date published
- 08/01/2019
- Academic Unit
- Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Orthodontics; Anatomy and Cell Biology; Pharmaceutical Sciences and Experimental Therapeutics; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9984025468602771
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