Journal article
MicroRNA-200c Release from Gelatin-Coated 3D-Printed PCL Scaffolds Enhances Bone Regeneration
ACS biomaterials science & engineering, Vol.10(4), pp.2337-2350
04/08/2024
DOI: 10.1021/acsbiomaterials.3c01105
PMCID: PMC11005014
PMID: 38531043
Appears in UI Libraries Support Open Access
Abstract
The fabrication of clinically relevant synthetic bone grafts relies on combining multiple biodegradable biomaterials to create a structure that supports the regeneration of defects while delivering osteogenic biomolecules that enhance regeneration. MicroRNA-200c (miR-200c) functions as a potent osteoinductive biomolecule to enhance osteogenic differentiation and bone formation; however, synthetic tissue-engineered bone grafts that sustain the delivery of miR-200c for bone regeneration have not yet been evaluated. In this study, we created novel, multimaterial, synthetic bone grafts from gelatin-coated 3D-printed polycaprolactone (PCL) scaffolds. We attempted to optimize the release of pDNA encoding miR-200c by varying gelatin types, concentrations, and polymer crosslinking materials to improve its functions for bone regeneration. We revealed that by modulating gelatin type, coating material concentration, and polymer crosslinking, we effectively altered the release rates of pDNA encoding miR-200c, which promoted osteogenic differentiation in vitro and bone regeneration in a critical-sized calvarial bone defect animal model. We also demonstrated that crosslinking the gelatin coatings on the PCL scaffolds with low-concentration glutaraldehyde was biocompatible and increased cell attachment. These results strongly indicate the potential use of gelatin-based systems for pDNA encoding microRNA delivery in gene therapy and further demonstrate the effectiveness of miR-200c for enhancing bone regeneration from synthetic bone grafts.
Details
- Title: Subtitle
- MicroRNA-200c Release from Gelatin-Coated 3D-Printed PCL Scaffolds Enhances Bone Regeneration
- Creators
- Matthew T Remy - University of IowaChawin Upara - University of IowaQiong J Ding - University of IowaJacob M Miszuk - University of IowaHongli Sun - University of IowaLiu Hong - University of Iowa
- Resource Type
- Journal article
- Publication Details
- ACS biomaterials science & engineering, Vol.10(4), pp.2337-2350
- DOI
- 10.1021/acsbiomaterials.3c01105
- PMID
- 38531043
- PMCID
- PMC11005014
- NLM abbreviation
- ACS Biomater Sci Eng
- eISSN
- 2373-9878
- Publisher
- American Chemical Society
- Grant note
- DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: F31DE031153, R01DE026433, R01DE029159, T90DE023520
- Language
- English
- Electronic publication date
- 03/26/2024
- Date published
- 04/08/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
- Record Identifier
- 9984577033602771
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