Journal article
MicroRNA-200c Represses IL-6, IL-8, and CCL-5 Expression and Enhances Osteogenic Differentiation
PloS one, Vol.11(8), pp.e0160915-e0160915
2016
DOI: 10.1371/journal.pone.0160915
PMCID: PMC4987006
PMID: 27529418
Abstract
MicroRNAs (miRs) regulate inflammation and BMP antagonists, thus they have potential uses as therapeutic reagents. However, the molecular function of miR-200c in modulating proinflammatory and bone metabolic mediators and osteogenic differentiation is not known. After miR-200c was transduced into a human embryonic palatal mesenchyme (HEPM) (a cell line of preosteoblasts), using lentiviral vectors, the resulting miR-200c overexpression increased osteogenic differentiation biomarkers, including osteocalcin (OCN) transcripts and calcium content. miR-200c expression also down-regulated interleukin (IL)-6, IL-8, and chemokine (C-C motif) ligand (CCL)-5 under lipopolysaccharide (LPS) stimulation and increased osteoprotegerin (OPG) in these cells. miR-200c directly regulates the expression of IL-6, IL-8 and CCL-5 transcripts by binding to their 3'UTRs. A plasmid-based miR-200c inhibitor effectively reduces their binding activities. Additionally, miR-200c delivered using polyethylenimine (PEI) nanoparticles effectively inhibits IL-6, IL-8 and CCL-5 in primary human periodontal ligament fibroblasts and increases the biomarkers of osteogenic differentiation in human bone marrow mesenchymal stem cells (MSCs), including calcium content, ALP, and Runx2. These data demonstrate that miR-200c represses IL-6, IL-8 and CCL-5 and improves osteogenic differentiation. miR-200c may potentially be used as an effective means to prevent periodontitis-associated bone loss by arresting inflammation and osteoclastogenesis and enhancing bone regeneration.
Details
- Title: Subtitle
- MicroRNA-200c Represses IL-6, IL-8, and CCL-5 Expression and Enhances Osteogenic Differentiation
- Creators
- Liu Hong - Center for Craniofacial Anomalies Research, Carver College of Medicine, the University of Iowa, Iowa City, IA, United States of AmericaThad Sharp - Department of Anatomy and Cell Biology, Carver College of Medicine, the University of Iowa, Iowa City, IA, United States of AmericaBehnoush Khorsand - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, the University of Iowa, Iowa City, IA, United States of AmericaCarol Fischer - Dows Institute for Dental Research, College of Dentistry, the University of Iowa, Iowa City, IA, United States of AmericaSteven Eliason - Department of Anatomy and Cell Biology, Carver College of Medicine, the University of Iowa, Iowa City, IA, United States of AmericaAli Salem - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, the University of Iowa, Iowa City, IA, United States of AmericaAdil Akkouch - Dows Institute for Dental Research, College of Dentistry, the University of Iowa, Iowa City, IA, United States of AmericaKim Brogden - Dows Institute for Dental Research, College of Dentistry, the University of Iowa, Iowa City, IA, United States of AmericaBrad A Amendt - Department of Anatomy and Cell Biology, Carver College of Medicine, the University of Iowa, Iowa City, IA, United States of America
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.11(8), pp.e0160915-e0160915
- DOI
- 10.1371/journal.pone.0160915
- PMID
- 27529418
- PMCID
- PMC4987006
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science; United States
- Grant note
- R21 DE024799 / NIDCR NIH HHS
- Language
- English
- Date published
- 2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthodontics; Anatomy and Cell Biology; Pharmaceutical Sciences and Experimental Therapeutics; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Periodontics
- Record Identifier
- 9984025471602771
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