Journal article
MicroRNA-31 inhibits RhoA-mediated tumor invasion and chemotherapy resistance in MKN-45 gastric adenocarcinoma cells
Experimental biology and medicine (Maywood, N.J.), Vol.242(18), pp.1842-1847
08/24/2017
DOI: 10.1177/1535370217728460
PMCID: PMC5714140
PMID: 28836853
Abstract
microRNAs are small single-stranded non-coding RNA molecules which modify gene expression by silencing potential target genes. The aberrant expression of RhoA, a small GTPase protein of Rho family, is involved in gastric cancer tumorigenesis. Since miR-31 is a pleomorphic molecule, we evaluated the miR-31/RhoA axis in inducing the malignant phenotype of gastric cancer cells MKN-45. Also, the clinicopathological significance of RhoA was investigated in a well-defined collection of gastric carcinomas which were embedded in tissue microarray blocks. Induction of miR-31 in MKN-45 followed by suppression of RhoA expression resulted in increased sensitivity to 5-fluorouracil, inhibition of cell proliferation, and invasion compared to the control groups. Immunohistochemical analysis in gastric adenocarcinoma patients’ samples showed significantly higher expression of RhoA in diffuse versus intestinal subtype tumors (
P
= 0.009), poorly differentiated versus well and moderately differentiated tumors (
P
= 0.03) and the presence of vascular invasion versus the absence of vascular invasion (
P
= 0.04). Our findings suggest a critical role for miR-31, as a tumor suppressor gene, in gastric cancer tumorigenesis by targeting the RhoA.
Details
- Title: Subtitle
- MicroRNA-31 inhibits RhoA-mediated tumor invasion and chemotherapy resistance in MKN-45 gastric adenocarcinoma cells
- Creators
- Alireza Korourian - Iran University of Medical SciencesRaheleh Roudi - Iran University of Medical SciencesAhmad Shariftabrizi - University at Buffalo, State University of New YorkZahra Madjd - Iran University of Medical Sciences
- Resource Type
- Journal article
- Publication Details
- Experimental biology and medicine (Maywood, N.J.), Vol.242(18), pp.1842-1847
- Publisher
- SAGE Publications
- DOI
- 10.1177/1535370217728460
- PMID
- 28836853
- PMCID
- PMC5714140
- ISSN
- 1535-3702
- eISSN
- 1535-3699
- Language
- English
- Date published
- 08/24/2017
- Academic Unit
- Radiology
- Record Identifier
- 9984318820402771
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