Journal article
MicroRNA Expression Profiles in External Cervical Resorption
Journal of endodontics, Vol.45(9), pp.1106-1113
09/01/2019
DOI: 10.1016/j.joen.2019.06.001
PMCID: PMC7188310
PMID: 31351582
Abstract
Introduction: External cervical resorption (ECR) has been challenging for its diagnosis, prevention, and treatment. Its etiology and pathogenesis are largely unknown. This study characterized microRNA (miRNA) expression patterns of human tissues from ECR lesions and identified potential messenger RNA targets and pathways. Methods: Granulomatous tissues from ECR (n = 5) and their adjacent nonaffected asymptomatic gingival connective tissues (n = 5) were collected. Similarly, chronic periodontitis (CP) and control samples were collected (n = 3). Quantitative reverse transcription polymerase chain reaction array analysis compared the expression profiles of 88 miRNAs between diseases. Differentially expressed miRNAs were identified using the Student t test. Bioinformatics for messenger RNA (miRWalk) and KEGG pathway analyses were performed to identify predicted target genes and biological/cellular functions and signaling pathways. Results: Three miRNAs (miR-20a-5p, miR-210-3p, and miR-99a-4p) were significantly down-regulated and 1 miRNA (miR-122-5p) was significantly up-regulated in ECR (P < .05). One up-regulated and 1 down-regulated miRNA reached the significance threshold in CP. A comparison of miRNA expression in ECR and CP identified 3 differentially expressed miRNAs, indicating differences in disease pathobiology. Inflammation-associated Wnt, PI3K-Akt, mitogen-activated protein kinases signaling, and bone formation-associated transforming growth factor beta pathways were identified and predicted to be modulated by differentially expressed miRNAs in both ECR and CP. Biological processes unique to each disease entity were identified, such as T- and B-cell receptor signaling pathways, osteoclast differentiation, and extracellular matrix-receptor interaction for CP. Glycosaminoglycan biosynthesis, mineral absorption, and insulin signaling pathways for ECR were identified. Conclusions: This proof-of-principle in vivo study indicated that ECR has both common and unique miRNA expression profiles in comparison with CP, which are predicted to target genes regulating inflammation, immunity, and metabolism of mineralized tissues.
Details
- Title: Subtitle
- MicroRNA Expression Profiles in External Cervical Resorption
- Creators
- Mary T. Pettiette - University of North Carolina at Chapel HillShaoping Zhang - University of IowaAntonio J. Moretti - University of North Carolina at Chapel HillSteven J. Kim - Univ North Carolina Chapel Hill, Sch Dent, Dept Periodontol, Chapel Hill, NC 27599 USAAfsar R. Naqvi - University of Illinois ChicagoSalvador Nares - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Journal of endodontics, Vol.45(9), pp.1106-1113
- DOI
- 10.1016/j.joen.2019.06.001
- PMID
- 31351582
- PMCID
- PMC7188310
- NLM abbreviation
- J Endod
- ISSN
- 0099-2399
- eISSN
- 1878-3554
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- American Association of Endodontists Foundation R21 DE026259-01A1; R01 DE027980; R03 DE027147; R01 DE02105201A1; 1K99DE027086 / National Institutes of Health/National Institute of Dental and Craniofacial Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R03DE027147 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
- Language
- English
- Date published
- 09/01/2019
- Academic Unit
- Periodontics
- Record Identifier
- 9984367718102771
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