MicroRNA modulation in obesity and periodontitis

R Perri; S Nares; S Zhang; S P Barros; S Offenbacher

doi:10.1177/0022034511425045

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MicroRNA modulation in obesity and periodontitis

Journal article

Open access

Peer reviewed

MicroRNA modulation in obesity and periodontitis

R Perri, S Nares, S Zhang, S P Barros and S Offenbacher

Journal of dental research, Vol.91(1), pp.33-38

01/2012

DOI: 10.1177/0022034511425045

PMCID: PMC3232116

PMID: 22043006

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https://europepmc.org/articles/pmc3232116View

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Abstract

The aim of this pilot investigation was to determine if microRNA expression differed in the presence or absence of obesity, comparing gingival biopsies obtained from patients with or without periodontal disease. Total RNA was extracted from gingival biopsy samples collected from 20 patients: 10 non-obese patients (BMI < 30 kg/m(2)) and 10 obese patients (BMI > 30 kg/m(2)), each group with 5 periodontally healthy sites and 5 chronic periodontitis sites. MicroRNA expression patterns were assessed with a quantitative microRNA PCR array to survey 88 candidate microRNA species. Four microRNA databases were used to identify potential relevant mRNA target genes of differentially expressed microRNAs. Two microRNA species (miR-18a, miR-30e) were up-regulated among obese individuals with a healthy periodontium. Two microRNA species (miR-30e, miR-106b) were up-regulated in non-obese individuals with periodontal disease. In the presence of periodontal disease and obesity, 9 of 11 listed microRNAs were significantly up-regulated (miR-15a, miR-18a, miR-22, miR-30d, miR-30e, miR-103, miR-106b, miR-130a, miR-142-3p, miR-185, and miR-210). Predicted targets include 69 different mRNAs from genes that comprise cytokines, chemokines, specific collagens, and regulators of glucose and lipid metabolism. The expression of specific microRNA species in obesity, which could also target and post-transcriptionally modulate cytokine mRNA, provides new insight into possible mechanisms of how risk factors might modify periodontal inflammation and may represent novel therapeutic targets.

Up-Regulation

Humans

Middle Aged

Carbohydrate Metabolism - genetics

Male

Obesity - genetics

Case-Control Studies

Young Adult

Chronic Periodontitis - genetics

Adult

Female

Lipid Metabolism - genetics

Cytokines - genetics

Chronic Periodontitis - complications

PPAR gamma - genetics

Fibrillar Collagens - genetics

Interleukin-6 - genetics

Obesity - complications

Gene Expression Regulation

MicroRNAs - biosynthesis

Chemokines - genetics

PPAR gamma - biosynthesis

Pilot Projects

NF-kappa B - genetics

NF-kappa B - biosynthesis

Adolescent

Interleukin-6 - biosynthesis

Aged

MicroRNAs - genetics

Details

Title: Subtitle: MicroRNA modulation in obesity and periodontitis
Creators: R Perri - Department of Periodontology and School of Dentistry, University of North Carolina at Chapel Hill, NC, USA
S Nares
S Zhang
S P Barros
S Offenbacher
Resource Type: Journal article
Publication Details: Journal of dental research, Vol.91(1), pp.33-38
Publisher: United States
DOI: 10.1177/0022034511425045
PMID: 22043006
PMCID: PMC3232116
ISSN: 0022-0345
eISSN: 1544-0591
Grant note: UL1-RR025747 / NCRR NIH HHS\nR01 DE021052 / NIDCR NIH HHS\n1R01DE021052-01 / NIDCR NIH HHS\nUL1 RR025747 / NCRR NIH HHS
Language: English
Date published: 01/2012
Academic Unit: Periodontics
Record Identifier: 9984065991102771

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