Journal article
MicroRNAs Play a Critical Role in Tooth Development
Journal of dental research, Vol.89(8), pp.779-784
08/2010
DOI: 10.1177/0022034510369304
PMCID: PMC3014323
PMID: 20505045
Abstract
MicroRNAs are known to regulate gene function in many tissues and organs, but their expression and function, if any, in tooth development are elusive. We sought to identify them by microRNA screening analyses and reveal their overall roles by inactivating
Dicer1
in the dental epithelium and mesenchyme. Discrete sets of microRNAs are expressed in molars compared with incisors as well as epithelium compared with mesenchyme. Conditional knockout (cKO) of
Dicer1
(mature microRNAs) in the dental epithelium of the
Pitx2-Cre
mouse results in multiple and branched enamel-free incisors and cuspless molars, and change in incisor patterning and in incisor and molar size and shape. Analyses of differentiating dental epithelial markers reveal a defect in ameloblast differentiation. Conversely, the cervical loop (stem cell niche) is expanded in
Dicer1
cKO. These results demonstrate that tooth development is tightly controlled by microRNAs and that specific microRNAs regulate tooth epithelial stem cell differentiation.
Details
- Title: Subtitle
- MicroRNAs Play a Critical Role in Tooth Development
- Creators
- H Cao - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAJ Wang - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAS.R Venugopalan - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAS Elangovan - Department of Biomineralization, The Forsyth Institute, Boston, MA, USAX Li - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAB.A Amendt - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAS Florez - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAZ Huang - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USAZ Skobe - Department of Biomineralization, The Forsyth Institute, Boston, MA, USAH.C Margolis - Department of Biomineralization, The Forsyth Institute, Boston, MA, USAJ.F Martin - Institute of Biosciences and Technology, Texas A&M Health Science Center, 2121 W. Holcombe Boulevard, Houston, TX 77030, USA
- Resource Type
- Journal article
- Publication Details
- Journal of dental research, Vol.89(8), pp.779-784
- DOI
- 10.1177/0022034510369304
- PMID
- 20505045
- PMCID
- PMC3014323
- NLM abbreviation
- J Dent Res
- ISSN
- 0022-0345
- eISSN
- 1544-0591
- Publisher
- SAGE Publications; Sage CA: Los Angeles, CA
- Language
- English
- Date published
- 08/2010
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthodontics; Anatomy and Cell Biology; Endodontics; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Periodontics
- Record Identifier
- 9984065703302771
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