Microarray analysis identifies differentiation-associated genes regulated by human papillomavirus type 16 E6

Carol L Duffy; Stacia L Phillips; Aloysius J Klingelhutz

doi:10.1016/S0042-6822(03)00390-8

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Microarray analysis identifies differentiation-associated genes regulated by human papillomavirus type 16 E6

Journal article

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Peer reviewed

Microarray analysis identifies differentiation-associated genes regulated by human papillomavirus type 16 E6

Carol L Duffy, Stacia L Phillips and Aloysius J Klingelhutz

Virology (New York, N.Y.), Vol.314(1), pp.196-205

2003

DOI: 10.1016/S0042-6822(03)00390-8

PMID: 14517073

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Abstract

In this study, we used oligonucleotide microarray analysis to determine which cellular genes are regulated by the human papillomavirus type 16 (HPV-16) E6 oncoprotein. We found that E6 causes the downregulation of a large number of cellular genes involved in keratinocyte differentiation, including genes such as small proline-rich proteins, transglutaminase, involucrin, elafin, and cytokeratins, which are normally involved in the production of the cornified cell envelope. In contrast, E6 upregulates several genes, such as vimentin, that are usually expressed in mesenchymal lineages. E6 also modulates levels of genes involved in inflammation, including Cox-1 and Nag-1. By using E6 mutants that differentially target p53 for degradation, we determined that E6 regulates cellular genes by both p53-dependent and independent mechanisms. The microarray data also indicate that HPV-16 E6 modulates certain effects of HPV-16 E7 on cellular gene expression. The identification of E6-regulated genes in this analysis provides a basis for further studies on their role in HPV infection and cellular transformation.

Cornified cell envelope

Human papillomavirus

E6 and E7 oncogenes

Keratinocyte

Differentiation

Microarray

Telomerase

HPV

p53

Details

Title: Subtitle: Microarray analysis identifies differentiation-associated genes regulated by human papillomavirus type 16 E6
Creators: Carol L Duffy - Department of Microbiology and Holden Cancer Center, University of Iowa, 2202 MEBRF, 375 Newton Road, Iowa City, IA 52242, USA
Stacia L Phillips - Department of Microbiology and Holden Cancer Center, University of Iowa, 2202 MEBRF, 375 Newton Road, Iowa City, IA 52242, USA
Aloysius J Klingelhutz - Department of Microbiology and Holden Cancer Center, University of Iowa, 2202 MEBRF, 375 Newton Road, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.314(1), pp.196-205
DOI: 10.1016/S0042-6822(03)00390-8
PMID: 14517073
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 2003
Academic Unit: Microbiology and Immunology; Anatomy and Cell Biology; Radiation Oncology
Record Identifier: 9984001115202771

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