Microarray analysis of the effect of dexamethasone on murine cochlear explants

Yukihide Maeda; Kunihiro Fukushima; Misato Hirai; Shin Kariya; Richard J.H Smith; Kazunori Nishizaki

doi:10.3109/00016489.2010.498836

Back

Microarray analysis of the effect of dexamethasone on murine cochlear explants

Journal article

Open access

Peer reviewed

Microarray analysis of the effect of dexamethasone on murine cochlear explants

Yukihide Maeda, Kunihiro Fukushima, Misato Hirai, Shin Kariya, Richard J.H Smith and Kazunori Nishizaki

Acta oto-laryngologica, Vol.130(12), pp.1329-1334

12/2010

DOI: 10.3109/00016489.2010.498836

PMCID: PMC2981696

PMID: 20735180

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/2981696View

Open Access

Abstract

Abstract Conclusions: The microarray analysis identified 39 genes up- or down-regulated by dexamethasone in the cultured tissue of mice cochlea. Of the eight genes most highly affected, several are suggested to have protective effects in the traumatized inner ear (Fkbp5, Glucocorticoid-induced leucine zipper (Gilz), glutathione peroxidase 3) and for others, a plausible mechanism of action can be offered (claudin 10, glutamate-ammonia ligase). The present data may support the use of dexamethasone to treat acute sensorineural hearing loss. It is warrantable to test these results in the in vivo cochlea. Objectives: To identify genes whose expression is markedly up- or down-regulated by dexamethasone in the cochlear tissue. Methods: Murine cochlear tissue was cultured with or without dexamethasone for 48 h in vitro. The gene expression profiles were compared between the dexamethasone-treated and untreated cochlear tissue using a microarray that covers 33 696 transcripts (24 878 genes) of mice and quantitative real-time RT-PCR. Results: The microarray analysis identified 39 genes that are up- or down-regulated by more than twofold in the presence of dexamethasone in the cochlear culture. Genes up- or down-regulated by at least threefold include Fkbp5, Gilz, glutathione peroxidase 3, claudin 10, glutamate-ammonia ligase, proteoglycan 1, integrin beta-like 1, and alpha subunit of glycoprotein hormone.

Details

Title: Subtitle: Microarray analysis of the effect of dexamethasone on murine cochlear explants
Creators: Yukihide Maeda - 1Department of Otolaryngology - Head and Neck Surgery, Okayama University Graduate School of Medicine, Okayama, Japan
Kunihiro Fukushima - 1Department of Otolaryngology - Head and Neck Surgery, Okayama University Graduate School of Medicine, Okayama, Japan
Misato Hirai - 1Department of Otolaryngology - Head and Neck Surgery, Okayama University Graduate School of Medicine, Okayama, Japan
Shin Kariya - 1Department of Otolaryngology - Head and Neck Surgery, Okayama University Graduate School of Medicine, Okayama, Japan
Richard J.H Smith - 2Molecular Otolaryngology Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, Interdepartmental PhD Genetics Program, University of Iowa, Iowa City, IA, USA
Kazunori Nishizaki - 1Department of Otolaryngology - Head and Neck Surgery, Okayama University Graduate School of Medicine, Okayama, Japan
Resource Type: Journal article
Publication Details: Acta oto-laryngologica, Vol.130(12), pp.1329-1334
DOI: 10.3109/00016489.2010.498836
PMID: 20735180
PMCID: PMC2981696
NLM abbreviation: Acta Otolaryngol
ISSN: 0001-6489
eISSN: 1651-2251
Publisher: Informa Healthcare
Language: English
Date published: 12/2010
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Otolaryngology; Internal Medicine
Record Identifier: 9984006312702771

Metrics

32 Record Views

12 Times Cited - Web of Science