Journal article
Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression
Clinical & experimental metastasis, Vol.24(7), pp.551-565
2007
DOI: 10.1007/s10585-007-9092-8
PMCID: PMC2214901
PMID: 17896182
Abstract
We used Affymetrix oligonucleotide microarrays to compare gene expression profiles of the metastatic parental breast cancer cell line MDA-MB-435 (435) and the non-metastatic daughter cell line created by the stable expression of the BReast cancer Metastasis Suppressor 1 (
BRMS1
) gene in 435 cells, MDA-MB-435-BRMS1 (435/BRMS1). Analysis of microarray data provided insight into some of the potential mechanisms by which
BRMS1
inhibits tumor formation at secondary sites. Furthermore, due to the importance of the microenvironment, we also examined gene expression under different growth conditions (i.e., plus or minus serum), however, expression of 565 genes was significantly (adjusted
p
-value <0.05) altered regardless of
in vitro
growth conditions. BRMS1 expression significantly increased multiple major histocompatability complex (MHC) genes and significantly decreased expression of several genes associated with protein localization and secretion. The pattern of gene expression associated with BRMS1 expression suggests that metastasis suppression may be mediated by enhanced immune recognition, altered transport, and/or secretion of metastasis-associated proteins.
Details
- Title: Subtitle
- Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression
- Creators
- Patricia J Champine - Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700, USAJacob Michaelson - Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700, USABart C Weimer - Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700, USADanny R Welch - Department of Pathology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294-0019, USADaryll B DeWald - National Foundation for Cancer Research, Center for Metastasis Research, Logan, Utah 84322-5305, USA
- Resource Type
- Journal article
- Publication Details
- Clinical & experimental metastasis, Vol.24(7), pp.551-565
- DOI
- 10.1007/s10585-007-9092-8
- PMID
- 17896182
- PMCID
- PMC2214901
- ISSN
- 0262-0898
- eISSN
- 1573-7276
- Language
- English
- Date published
- 2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Communication Sciences and Disorders; Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984070523102771
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