Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression

Patricia J Champine; Jacob Michaelson; Bart C Weimer; Danny R Welch; Daryll B DeWald

doi:10.1007/s10585-007-9092-8

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Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression

Journal article

Open access

Peer reviewed

Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression

Patricia J Champine, Jacob Michaelson, Bart C Weimer, Danny R Welch and Daryll B DeWald

Clinical & experimental metastasis, Vol.24(7), pp.551-565

2007

DOI: 10.1007/s10585-007-9092-8

PMCID: PMC2214901

PMID: 17896182

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https://doi.org/10.1007/s10585-007-9092-8View

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Abstract

We used Affymetrix oligonucleotide microarrays to compare gene expression profiles of the metastatic parental breast cancer cell line MDA-MB-435 (435) and the non-metastatic daughter cell line created by the stable expression of the BReast cancer Metastasis Suppressor 1 ( BRMS1 ) gene in 435 cells, MDA-MB-435-BRMS1 (435/BRMS1). Analysis of microarray data provided insight into some of the potential mechanisms by which BRMS1 inhibits tumor formation at secondary sites. Furthermore, due to the importance of the microenvironment, we also examined gene expression under different growth conditions (i.e., plus or minus serum), however, expression of 565 genes was significantly (adjusted p -value <0.05) altered regardless of in vitro growth conditions. BRMS1 expression significantly increased multiple major histocompatability complex (MHC) genes and significantly decreased expression of several genes associated with protein localization and secretion. The pattern of gene expression associated with BRMS1 expression suggests that metastasis suppression may be mediated by enhanced immune recognition, altered transport, and/or secretion of metastasis-associated proteins.

breast cancer

MHC

microarray

BRMS1

Affymetrix

metastasis

Details

Title: Subtitle: Microarray analysis reveals potential mechanisms of BRMS1-mediated metastasis suppression
Creators: Patricia J Champine - Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700, USA
Jacob Michaelson - Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700, USA
Bart C Weimer - Center for Integrated BioSystems, Utah State University, Logan, Utah 84322-4700, USA
Danny R Welch - Department of Pathology, Comprehensive Cancer Center, University of Alabama at Birmingham, Birmingham, AL 35294-0019, USA
Daryll B DeWald - National Foundation for Cancer Research, Center for Metastasis Research, Logan, Utah 84322-5305, USA
Resource Type: Journal article
Publication Details: Clinical & experimental metastasis, Vol.24(7), pp.551-565
DOI: 10.1007/s10585-007-9092-8
PMID: 17896182
PMCID: PMC2214901
NLM abbreviation: Clin Exp Metastasis
ISSN: 0262-0898
eISSN: 1573-7276
Language: English
Date published: 2007
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Communication Sciences and Disorders; Psychiatry; Iowa Neuroscience Institute
Record Identifier: 9984070523102771

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