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Microbial Indicators of Dental Health, Dysbiosis, and Early Childhood Caries
Journal article   Open access   Peer reviewed

Microbial Indicators of Dental Health, Dysbiosis, and Early Childhood Caries

D Kahharova, V. Y Pappalardo, M. J Buijs, R. X de Menezes, M Peters, R Jackson, A. T Hara, G Eckert, B Katz, M. A Keels, …
Journal of dental research / International Association for Dental Research, and American Association for Dental Research, Vol.102(7), pp.759-766
07/2023
DOI: 10.1177/00220345231160756
PMCID: PMC10288166
PMID: 37042041
url
https://doi.org/10.1177/00220345231160756View
Published (Version of record) Open Access

Abstract

Dental caries lesions are a clinical manifestation of disease, preceded by microbial dysbiosis, which is poorly characterized and thought to be associated with saccharolytic taxa. Here, we assessed the associations between the oral microbiome of children and various caries risk factors such as demographics and behavioral and clinical data across early childhood and characterized over time the salivary and dental plaque microbiome of children before clinical diagnosis of caries lesions. Children (N = 266) were examined clinically at ~1, 2.5, 4, and 6.5 y of age. The microbiome samples were collected at 1, 2.5, and 4 y. Caries groups consisted of children who remained caries free (International Caries Detection and Assessment System [ICDAS] = 0) at all time points (CFAT) (n = 50); children diagnosed with caries (ICDAS ≥ 1) at 6.5 y (C6.5), 4 y (C4), or 2.5 y of age (C2.5); and children with early caries or advanced caries lesions at specific time points. Microbial community analyses were performed on zero-radius operational taxonomic units (zOTUs) obtained from V4 of 16S ribosomal RNA gene amplicon sequences. The oral microbiome of the children was affected by various factors, including antibiotic use, demographics, and dietary habits of the children and their caregivers. At all time points, various risk factors explained more of the variation in the dental plaque microbiome than in saliva. At 1 y, composition of saliva of the C4 group differed from that of the CFAT group, while at 2.5 y, this difference was observed only in plaque. At 4 y, multiple salivary and plaque zOTUs of genera Prevotella and Leptotrichia were significantly higher in samples of the C6.5 group than those of the CFAT group. In conclusion, up to 3 y prior to clinical caries detection, the oral microbial communities were already in a state of dysbiosis that was dominated by proteolytic taxa. Plaque discriminated dysbiotic oral ecosystems from healthy ones better than saliva.
 Antibiotics Dental Caries 16S rRNA child dental plaque saliva

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