Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

Timothy E Mattes; Jessica M Ewald; Yi Liang; Andres Martinez; Andrew M Awad; Keri C Hornbuckle; Jerald L Schnoor

doi:10.1016/j.dib.2021.107546

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Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

Journal article

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Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets

Timothy E Mattes, Jessica M Ewald, Yi Liang, Andres Martinez, Andrew M Awad, Keri C Hornbuckle and Jerald L Schnoor

Data in brief, Vol.39, p.107546

12/2021

DOI: 10.1016/j.dib.2021.107546

PMCID: PMC8601980

PMID: 34820490

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Abstract

The potential for aerobic and anaerobic microbial natural attenuation of PCBs in freshwater sediments is described by PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets generated, in duplicate, from 27 sediment samples collected from a PCB-contaminated freshwater lagoon (54 samples total). Sediment samples were subjected to a hexane PCB extraction protocol and the concentrations of 209 PCB congeners were determined in hexane extracts by gas chromatography with a tandem mass spectrometry detection. DNA was extracted from sediments sediment samples and used for qPCR and 16S rRNA amplicon sequencing. The abundance of 16S rRNA genes (i.e., Dehalococcoides and putative dechlorinating Chloroflexi) and functional genes (i.e., reductive dehalogenase (rdhA) and biphenyl dioxygenase (bphA)) associated with aerobic and anaerobic PCB biodegradation, along with the total 16S rRNA genes abundance, was determined by SYBR green qPCR. The microbial community composition and structure in all sediment samples was obtained by 16S rRNA gene amplicon sequencing. Primers targeting the 16S rRNA gene V4 region were used to produce 16S rRNA gene amplicons that were sequencing with the high-throughput Illumina MiSeq platform and sequencing chemistry. The 16S rRNA gene sequencing dataset along with PCB congener and qPCR datasets included as metadata, could be reused in meta-analyses that aim to determine microbial community interactions in contaminated environments, and uncover relationships between microbial community structure and environmental variable (e.g., PCB congener concentrations).

16S rRNA gene sequencing

Dehalococcoides

Polychlorinated biphenyls

Quantitative PCR

Reductive dehalogenase genes

Reductive dehalogenation

Wastewater lagoon sediments

Details

Title: Subtitle: Microbial communities in polychlorinated biphenyl (PCB)-contaminated wastewater lagoon sediments: PCB congener, quantitative PCR, and 16S rRNA gene amplicon sequencing datasets
Creators: Timothy E Mattes - University of Iowa
Jessica M Ewald - University of Iowa
Yi Liang - University of Iowa
Andres Martinez - University of Iowa
Andrew M Awad - University of Iowa
Keri C Hornbuckle - University of Iowa
Jerald L Schnoor - University of Iowa
Resource Type: Journal article
Publication Details: Data in brief, Vol.39, p.107546
DOI: 10.1016/j.dib.2021.107546
PMID: 34820490
PMCID: PMC8601980
NLM abbreviation: Data Brief
ISSN: 2352-3409
eISSN: 2352-3409
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000066, name: National Institute of Environmental Health Sciences, award: P42ES013661; DOI: 10.13039/100000002, name: National Institutes of Health
Language: English
Date published: 12/2021
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; IIHR--Hydroscience and Engineering
Record Identifier: 9984197393502771

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