Journal article
Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs
Journal of investigative and clinical dentistry, Vol.10(4), pp.e12439-n/a
11/2019
DOI: 10.1111/jicd.12439
PMID: 31313892
Abstract
To investigate the relationship between the microbiota of periodontal pockets (PP) and root canals (RC) in dogs submitted to experimental periodontal disease (ExPD).
ExPD was induced by combining cotton and wire ligatures. After 125 days, microbiological samples were collected from PP and RC. Strains isolated from 19 teeth were submitted to DNA extraction, 16S rRNA gene amplification and gene sequencing. Pearson's χ
- and Fisher's exact tests and McNemar's test were used when appropriate.
The number of species in PP was greater than in RC, with prevalence of obligate anaerobes and Gram-negative bacteria. In the PP predominated Fusobacterium necrophorum, Porphyromonas gingivalis, Prevotella loescheii, Campylobacter gracilis and Veillonella parvula. In the RC samples, 9 had microbial growth, with predominance of the following genera: Staphylococcus, Streptococcus and Neisseria. Eight genera were common to both sites in the same tooth. PP presented a greater number of species than the RC. No significant difference was observed in the species found in PP and RC in the same tooth.
Microbial composition of the RC could be modulated by the presence of periodontal disease, especially in cases of severe periodontal destruction. RC microbiota was less complex and diverse than the PP.
Details
- Title: Subtitle
- Microbiota of periodontal pockets and root canals in induced experimental periodontal disease in dogs
- Creators
- Cícero R Gadê-Neto - Universidade Estadual de Campinas (UNICAMP)Ronaldo R Rodrigues - Universidade Estadual de Campinas (UNICAMP)Lidiane M Louzada - Universidade Estadual de Campinas (UNICAMP)Rodrigo Arruda-Vasconcelos - Universidade Estadual de Campinas (UNICAMP)Fabrício B Teixeira - University of IowaRenato C Viana Casarin - Universidade Estadual de Campinas (UNICAMP)Brenda P F A Gomes - Universidade Estadual de Campinas (UNICAMP)
- Resource Type
- Journal article
- Publication Details
- Journal of investigative and clinical dentistry, Vol.10(4), pp.e12439-n/a
- DOI
- 10.1111/jicd.12439
- PMID
- 31313892
- ISSN
- 2041-1618
- eISSN
- 2041-1626
- Grant note
- CAPES - Finance Code 001 / Coordination for the Improvement of Higher Education Personnel Teaching, Research and Extension, State University of Campinas - UNICAMP (FAEPEX) FAPESP - 2015/23479-5 / São Paulo Research Foundation CNPq - 308162/2014-5 / National Council for Scientific and Technological Development
- Language
- English
- Date published
- 11/2019
- Academic Unit
- Endodontics
- Record Identifier
- 9984367737202771
Metrics
8 Record Views