Journal article
Microglia are required for protection against lethal coronavirus encephalitis in mice
The Journal of Clinical Investigation, Vol.128(3), pp.931-943
2018
DOI: 10.1172/JCI97229
PMCID: PMC5824854
PMID: 29376888
Abstract
Recent findings have highlighted the role of microglia in orchestrating normal development and refining neural network connectivity in the healthy CNS. Microglia are not only vital cells in maintaining CNS homeostasis, but also respond to injury, infection, and disease by undergoing proliferation and changes in transcription and morphology. A better understanding of the specific role of microglia in responding to viral infection is complicated by the presence of nonmicroglial myeloid cells with potentially overlapping function in the healthy brain and by the rapid infiltration of hematopoietic myeloid cells into the brain in diseased states. Here, we used an inhibitor of colony-stimulating factor 1 receptor (CSF1R) that depletes microglia to examine the specific roles of microglia in response to infection with the mouse hepatitis virus (MHV), a neurotropic coronavirus. Our results show that microglia were required during the early days after infection to limit MHV replication and subsequent morbidity and lethality. Additionally, microglia depletion resulted in ineffective T cell responses. These results reveal nonredundant, critical roles for microglia in the early innate and virus-specific T cell responses and for subsequent host protection from viral encephalitis.
Details
- Title: Subtitle
- Microglia are required for protection against lethal coronavirus encephalitis in mice
- Creators
- D. Lori Wheeler - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USAAlan Sariol - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USADavid K Meyerholz - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USAStanley Perlman - Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- The Journal of Clinical Investigation, Vol.128(3), pp.931-943
- DOI
- 10.1172/JCI97229
- PMID
- 29376888
- PMCID
- PMC5824854
- NLM abbreviation
- J Clin Invest
- ISSN
- 0021-9738
- eISSN
- 1558-8238
- Publisher
- American Society for Clinical Investigation
- Grant note
- RG 5340-A-7 / ;\nN36592 / ;
- Language
- English
- Date published
- 2018
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777350002771
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