Journal article
Microsporidia are related to Fungi: Evidence from the largest subunit of RNA polymerase II and other proteins
Proceedings of the National Academy of Sciences - PNAS, Vol.96(2), pp.580-585
01/19/1999
DOI: 10.1073/pnas.96.2.580
PMCID: PMC15179
PMID: 9892676
Abstract
We have determined complete gene sequences encoding the largest subunit of the RNA polymerase II (RBP1) from two Microsporidia, Vairimorpha necatrix and Nosema locustae. Phylogenetic analyses of these and other RPB1 sequences strongly support the notion that Microsporidia are not early-diverging eukaryotes but instead are specifically related to Fungi. Our reexamination of elongation factors EF-1α and EF-2 sequence data that had previously been taken as support for an early (Archezoan) divergence of these amitochondriate protists show such support to be weak and likely caused by artifacts in phylogenetic analyses. These EF data sets are, in fact, not inconsistent with a Microsporidia + Fungi relationship. In addition, we show that none of these proteins strongly support a deep divergence of Parabasalia and Metamonada, the other amitochondriate protist groups currently thought to compose early branches. Thus, the phylogenetic placement among eukaryotes for these protist taxa is in need of further critical examination.
Details
- Title: Subtitle
- Microsporidia are related to Fungi: Evidence from the largest subunit of RNA polymerase II and other proteins
- Creators
- Robert P HirtJohn M LogsdonBryan HealyMichael W DoreyW. Ford DoolittleT. Martin Embley
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.96(2), pp.580-585
- DOI
- 10.1073/pnas.96.2.580
- PMID
- 9892676
- PMCID
- PMC15179
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 01/19/1999
- Academic Unit
- Biology
- Record Identifier
- 9984217428602771
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