Journal article
Microtox® toxicity test: Detoxification of TNT and RDX contaminated solutions by poplar tissue cultures
Chemosphere (Oxford), Vol.71(10), pp.1970-1976
05/2008
DOI: 10.1016/j.chemosphere.2007.12.020
PMID: 18400248
Abstract
Poplar (Populus deltoides×nigra DN34) tissue cultures removed 2,4,6-trinitrotoluene (TNT) from an aqueous solution in five days, reducing the toxicity of the solution from highly toxic Microtox® EC value to that of the control. 1,3,5-Trinitro-1,3,5-triazacyclohexane (RDX) was taken up by the plant tissue cultures more slowly, but toxicity reduction of the solution was evident. The measurement of toxicity reduction of aqueous solutions containing TNT and RDX was performed using a novel methodology developed for use with the Microtox® testing system. Radiolabeled TNT and RDX were used to confirm removal of explosives from hydroponic solutions containing plant tissue cultures and to verify that toxicity did not change in solutions where no plant cultures were present (positive controls). High Performance Liquid Chromatography (HPLC) and Liquid Scintillation Counter (LSC) measurements confirmed removal of TNT and RDX from solutions containing poplar plant tissue cultures and constancy of the plant-free controls. In addition, metabolites were identified in remediated solutions by HPLC, confirming the mechanism by which plants can remediate groundwater, surface water, and soil solutions.
Details
- Title: Subtitle
- Microtox® toxicity test: Detoxification of TNT and RDX contaminated solutions by poplar tissue cultures
- Creators
- Brittany R Flokstra - The University of Iowa, 4105 Seamans Center, Iowa City, IA 52242, USABenoit Van Aken - West Virginia University, USAJerald L Schnoor - The University of Iowa, 4105 Seamans Center, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Chemosphere (Oxford), Vol.71(10), pp.1970-1976
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.chemosphere.2007.12.020
- PMID
- 18400248
- ISSN
- 0045-6535
- eISSN
- 1879-1298
- Language
- English
- Date published
- 05/2008
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9983991987402771
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