Journal article
Migrating Progenitor Cells Derived From Injured Cartilage Surface Respond to Damage-Associated Molecular Patterns
Cartilage, Vol.13(2_SUPPL), pp.755S-765S
12/01/2021
DOI: 10.1177/19476035211049559
PMCID: PMC8804768
PMID: 34636628
Abstract
Objective: To delineate the response of migrating chondrogenic progenitor cells (CPCs) that arose from the surface of mechanically injured articular cartilage to proinflammatory damage-associated-molecular-patterns (DAMPs). Design: Bovine CPCs and non-CPC chondrocytes isolated from either impacted or scratched articular cartilage were studied. Those 2 types of cells were treated with mitochondrial DAMPs (MTDs; 10 nM fMLF and 10 mu g/mL CpG DNA), or 10 nM HMGB1, or 10 ng/mL IL-1b for 24 hours. At the end of experiments, conditioned media and cell lysates were collected for analysis of expression levels of matrix metalloproteinases (MMPs), chemokines, and cytokines that are associated with cartilage degeneration with Western blotting and quantitative polymerase chain reaction. The difference of expression levels was compared by Welch's t-test. Results: Our data indicated that HMGB1 and MTDs remarkably upregulated pro-MMP-13 expression in CPCs. Compared with non-CPCs, CPCs expressed significantly more baseline mRNAs of MMP-13, CXCL12, and IL-6. MTDs greatly increased the expression of MMP-13 and IL-6 in CPCs by over 100-fold (P < 0.001). MTDs also significantly increased IL-8 expression in CPCs to a similar extent (P < 0.001). However, when IL-1b was present, CPCs expressed less MMP-3 and active MMP-13 proteins as well as less CCL2 and IL-6 than did non-CPCs. Conclusions: We concluded that CPCs were more sensitive than non-CPCs in response to DAMPs, especially MTDs. The proinflammatory nature of CPCs implied their critical role in the early phase of posttraumatic osteoarthritis development.
Details
- Title: Subtitle
- Migrating Progenitor Cells Derived From Injured Cartilage Surface Respond to Damage-Associated Molecular Patterns
- Creators
- Lei Ding - Jiangnan UniversityCheng Zhou - University of Iowa Hospitals and ClinicsHongjun Zheng - University of Iowa Hospitals and ClinicsQuanming Wang - Jiangnan UniversityHaiyan Song - Second Affiliated Hospital of Harbin Medical UniversityJoseph A. Buckwalter - University of IowaJames A. Martin - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cartilage, Vol.13(2_SUPPL), pp.755S-765S
- DOI
- 10.1177/19476035211049559
- PMID
- 34636628
- PMCID
- PMC8804768
- NLM abbreviation
- Cartilage
- ISSN
- 1947-6035
- eISSN
- 1947-6043
- Publisher
- Sage
- Number of pages
- 11
- Grant note
- P50 AR055533 / US DHHS, National Institutes of Health/NIAMS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) BK20171143 / Jiangsu Provincial Natural Science Foundation of China; Natural Science Foundation of Jiangsu Province
- Language
- English
- Date published
- 12/01/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
- Record Identifier
- 9984304720402771
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