Journal article
Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection
Experimental neurology, Vol.221(1), pp.122-128
01/01/2010
DOI: 10.1016/j.expneurol.2009.10.010
PMID: 19853605
Abstract
Cell-based treatments have been considered a promising therapy for neurological diseases. However, currently there are no clinically available methods to monitor whether the transplanted cells reach and remain in the brain. In this study we investigated the feasibility of detecting the distribution and homing of autologous bone-marrow mononuclear cells (BMMCs) labeled with Technetium-99 m (Tc-99m) in a cell-based therapy clinical study for chronic ischemic stroke. Six male patients (ages 24-65 years) with ischemic cerebral infarcts within the middle cerebral artery (MCA) between 59 and 82 days were included. Cell dose ranged from 1.25 x 10(8) to 5 x 10(8). Approximately 2 x 10(7) cells were labeled with Tc-99m and intra-arterially delivered together with the unlabeled cells via a catheter navigated to the MCA. None of the patients showed any complications on the 120-day follow-up. Whole body scintigraphies indicated cell homing in the brain of all patients at 2 h, while the remaining uptake was mainly distributed to liver, lungs, spleen, kidneys and bladder. Moreover, quantification of uptake in Single-Photon Emission Computed Tomography (SPECF) at 2 h showed preferential accumulation of radioactivity in the hemisphere affected by the ischemic infarct in all patients. However, at 24 h homing could only distinguished in the brains of 2 patients, while in all patients uptake was still seen in the other organs. Taken together, these results indicate that labeling of BMMCs with Tc-99m is a safe and feasible technique that allows monitoring the migration and engraftment of intra-arterially transplanted cells for at least 24 h. (C) 2009 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Migration and homing of bone-marrow mononuclear cells in chronic ischemic stroke after intra-arterial injection
- Creators
- Lea Mirian Barbosa da Fonseca - Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Dept Radiol, Ilha Fundao, BR-21949913 Rio De Janeiro, BrazilBianca Gutfilen - Universidade Federal do Rio de JaneiroPaulo Henrique Rosado de Castro - Univ Fed Rio de Janeiro, Hosp Univ Clementino Fraga Filho, Dept Radiol, Ilha Fundao, BR-21949913 Rio De Janeiro, BrazilValeria Battistella - Universidade Federal do Rio de JaneiroRegina C. S. Goldenberg - Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949913 Rio De Janeiro, BrazilTais Kasai-Brunswick - Universidade Federal do Rio de JaneiroClaudia L. R. Chagas - Universidade Federal do Rio de JaneiroEduardo Wajnberg - Hospital Universitário Clementino Fraga FilhoAngelo Maiolino - Hospital Universitário Clementino Fraga FilhoSergio Salles Xavier - Universidade Federal do Rio de JaneiroCharles Andre - Universidade Federal do Rio de JaneiroRosalia Mendez-Otero - Universidade Federal do Rio de JaneiroGabriel R. de Freitas - Universidade Federal do Rio de Janeiro
- Resource Type
- Journal article
- Publication Details
- Experimental neurology, Vol.221(1), pp.122-128
- DOI
- 10.1016/j.expneurol.2009.10.010
- PMID
- 19853605
- NLM abbreviation
- Exp Neurol
- ISSN
- 0014-4886
- eISSN
- 1090-2430
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- 180.011/2005 / Fundacao Carlos Chagas Filho de Amparo A Pesquisa do Estado do Rio de Janeiro (FAPERJ); Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio De Janeiro (FAPERJ) 552201/2005-7 / Ministry of Health and Ministry of Science and Technology of Brazil 110.391/2007 / Rosalia Mendez-Otero
- Language
- English
- Date published
- 01/01/2010
- Academic Unit
- Radiology
- Record Identifier
- 9985143552602771
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