Migration of the Plastid Genome to the Nucleus in a Peridinin Dinoflagellate

Jeremiah D Hackett; Hwan Su Yoon; M. Bento Soares; Maria F Bonaldo; Thomas L Casavant; Todd E Scheetz; Tetyana Nosenko; Debashish Bhattacharya

doi:10.1016/j.cub.2004.01.032

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Migration of the Plastid Genome to the Nucleus in a Peridinin Dinoflagellate

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Migration of the Plastid Genome to the Nucleus in a Peridinin Dinoflagellate

Jeremiah D Hackett, Hwan Su Yoon, M. Bento Soares, Maria F Bonaldo, Thomas L Casavant, Todd E Scheetz, Tetyana Nosenko and Debashish Bhattacharya

Current Biology, Vol.14(3), pp.213-218

02/03/2004

DOI: 10.1016/j.cub.2004.01.032

PMID: 14761653

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Abstract

Dinoflagellate algae are important primary producers and of significant ecological and economic impact because of their ability to form “red tides”[1]. They are also models for evolutionary research because of an unparalleled ability to capture photosynthetic organelles (plastids) through endosymbiosis [2]. The nature and extent of the plastid genome in the dominant perdinin-containing dinoflagellates remain, however, two of the most intriguing issues in plastid evolution. The plastid genome in these taxa is reduced to single-gene minicircles [3, 4] encoding an incomplete (until now 15) set of plastid proteins. The location of the remaining photosynthetic genes is unknown. We generated a data set of 6,480 unique expressed sequence tags (ESTs) from the toxic dinoflagellate Alexandrium tamarense (for details, see the Experimental Procedures in the Supplemental Data) to find the missing plastid genes and to understand the impact of endosymbiosis on genome evolution. Here we identify 48 of the non-minicircle-encoded photosynthetic genes in the nuclear genome of A. tamarense, accounting for the majority of the photosystem. Fifteen genes that are always found on the plastid genome of other algae and plants have been transferred to the nucleus in A. tamarense. The plastid-targeted genes have red and green algal origins. These results highlight the unique position of dinoflagellates as the champions of plastid gene transfer to the nucleus among photosynthetic eukaryotes.

Details

Title: Subtitle: Migration of the Plastid Genome to the Nucleus in a Peridinin Dinoflagellate
Creators: Jeremiah D Hackett - Department of Biological Sciences and Center for Comparative Genomics, The University of Iowa, Iowa City, IA 52242 USA
Hwan Su Yoon - Department of Biological Sciences and Center for Comparative Genomics, The University of Iowa, Iowa City, IA 52242 USA
M. Bento Soares - Department of Pediatrics, The University of Iowa, Iowa City, IA 52242 USA
Maria F Bonaldo - Department of Pediatrics, The University of Iowa, Iowa City, IA 52242 USA
Thomas L Casavant - Department of Electrical and Computer Engineering, The University of Iowa, Iowa City, IA 52242 USA
Todd E Scheetz - Department of Ophthalmology and Center for Bioinformatics and Computational Biology, The University of Iowa, Iowa City, IA 52242 USA
Tetyana Nosenko - Department of Biological Sciences and Center for Comparative Genomics, The University of Iowa, Iowa City, IA 52242 USA
Debashish Bhattacharya - Department of Biological Sciences and Center for Comparative Genomics, The University of Iowa, Iowa City, IA 52242 USA
Resource Type: Journal article
Publication Details: Current Biology, Vol.14(3), pp.213-218
DOI: 10.1016/j.cub.2004.01.032
PMID: 14761653
NLM abbreviation: Curr Biol
ISSN: 0960-9822
eISSN: 1879-0445
Publisher: Elsevier Inc
Language: English
Date published: 02/03/2004
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Ophthalmology and Visual Sciences
Record Identifier: 9983979951702771

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