Mild disintegration of the green microalgae Chlorella vulgaris using bead milling

P. R. Postma; T. L. Miron; G. Olivieri; M. J. Barbosa; R. H. Wijffels; M. H. M. Eppink

doi:10.1016/j.biortech.2014.09.033

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Mild disintegration of the green microalgae Chlorella vulgaris using bead milling

Journal article

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Mild disintegration of the green microalgae Chlorella vulgaris using bead milling

P. R. Postma, T. L. Miron, G. Olivieri, M. J. Barbosa, R. H. Wijffels and M. H. M. Eppink

Bioresource technology, Vol.184, pp.297-304

05/01/2015

DOI: 10.1016/j.biortech.2014.09.033

PMID: 25280602

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https://research.wur.nl/en/publications/mild-disintegration-of-the-green-microalgae-chlorella-vulgaris-usView

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Abstract

In this work, the mild disintegration of the microalgae Chlorella vulgaris for the release of intracellular products has been studied. By means of bead milling the microalgae suspensions were successfully disintegrated at different biomass concentrations (25-145 g(DW) kg(-1)) over a range of agitator speeds (6-12 m s(-1)). In all cases over 97% of cell disintegration was achieved resulting in a release of water soluble proteins. A clear optimum rate of disintegration and protein release was observed at an agitator speed of 9-10 m s(-1) regardless of the biomass concentration. Selective extraction of water soluble proteins was observed as proteins released sooner than cell disintegration took place. Proteins could be released at 85% lower energy input than for cell disintegration resulting in specific energy consumptions well below 2.5 kWh kg(DW)(.)(1) (C) 2014 Elsevier Ltd. All rights reserved.

Agricultural Engineering

Agriculture

Biotechnology & Applied Microbiology

Energy & Fuels

Life Sciences & Biomedicine

Science & Technology

Technology

Details

Title: Subtitle: Mild disintegration of the green microalgae Chlorella vulgaris using bead milling
Creators: P. R. Postma - Wageningen University & Research
T. L. Miron - Wageningen University & Research
G. Olivieri - Wageningen University & Research
M. J. Barbosa - Univ Wageningen & Res Ctr, Food & Biobased Res, AlgaePARC, NL-6700 AA Wageningen, Netherlands
R. H. Wijffels - Wageningen University & Research
M. H. M. Eppink - Wageningen University & Research
Resource Type: Journal article
Publication Details: Bioresource technology, Vol.184, pp.297-304
DOI: 10.1016/j.biortech.2014.09.033
PMID: 25280602
NLM abbreviation: Bioresour Technol
ISSN: 0960-8524
eISSN: 1873-2976
Publisher: Elsevier
Number of pages: 8
Grant note: IPOP Biorefinery of Wageningen University and Research Centre
Language: English
Date published: 05/01/2015
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984397918202771

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