Journal article
Milking exopolysaccharides from Botryococcus braunii CCALA778 by membrane filtration
Algal research (Amsterdam), Vol.34, pp.175-181
09/01/2018
DOI: 10.1016/j.algal.2018.07.018
Abstract
The aim of this work was to optimize the efficiency of extraction and recovery, also known as 'milking', of exopolysaccharides (excreted polysaccharides, EPS) from continuous cultures of Botryococcus braunii CCALA778. First, an indoor process was developed and optimised, ensuring the highest milking efficiency without compromising culture viability. For this, photobioreactors were operated in a photo-chemostat mode under simulated outdoor conditions of a typical summer at AlgaePARC (51 degrees 59'44.1 '' N 5 degrees 39'26.2 '' E) in Wageningen, The Netherlands. Once a steady state was reached, areal productivities of 23 g m(-2) d(-1) and 3 g m(-2) d(-1) for biomass and EPS were achieved. EPS milking was done by membrane filtration of one reactor volume at the beginning of the dark period. After optimization, the maximum recovery of EPS, without damaging the cells, was 12%; yielding a daily EPS extraction rate of 0.36 g M-2 d(-1) . The optimised process was scaled-up and applied outdoors during the summer (at AlgaePARC facilities). Outdoor cultures showed 25% lower biomass productivity (17 g M-2 d(-1)) but an 25% higher EPS productivity (4 g M-2 d(-1)). The efficiency in the milking, however, decreased as compared to indoor results. Only 3% of the total content of EPS produced outdoors was milked (0.12 g m(-2)d(-1) ). To improve the EPS milking process, future research should focus on increasing the EPS extraction yield without negatively influencing its production by Botryococcus braunii.
Details
- Title: Subtitle
- Milking exopolysaccharides from Botryococcus braunii CCALA778 by membrane filtration
- Creators
- Rafael Garcia-Cubero - Wageningen University & ResearchWeiliang Wang - East China University of Science and TechnologyJudit Martin - Universidad de ValladolidElisabeth Bermejo - Universidad de HuelvaLolke Sijtsma - Wageningen University & ResearchArnoud Togtema - Wageningen University & ResearchMaria J. Barbosa - Wageningen Univ, Bioproc Engn, AlgaePARC, POB 16, NL-6700 AA Wageningen, NetherlandsDorinde M. M. Kleinegris - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Algal research (Amsterdam), Vol.34, pp.175-181
- DOI
- 10.1016/j.algal.2018.07.018
- ISSN
- 2211-9264
- eISSN
- 2211-9264
- Publisher
- Elsevier
- Number of pages
- 7
- Grant note
- 311956 / European Community; European Commission
- Language
- English
- Date published
- 09/01/2018
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984397923202771
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