Journal article
Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source
Lab on a chip, Vol.9(5), pp.733-736
01/01/2009
DOI: 10.1039/b810705j
PMID: 19224025
Abstract
A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (m-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol.
Details
- Title: Subtitle
- Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source
- Creators
- Kangning Ren - Tsinghua UniversityQionglin Liang - Tsinghua UniversityXuan Mu - East China University of Science and TechnologyGuoan Luo - Tsinghua UniversityYiming Wang - Tsinghua University
- Resource Type
- Journal article
- Publication Details
- Lab on a chip, Vol.9(5), pp.733-736
- Publisher
- Royal Soc Chemistry
- DOI
- 10.1039/b810705j
- PMID
- 19224025
- ISSN
- 1473-0197
- eISSN
- 1473-0189
- Number of pages
- 4
- Grant note
- 20475031; 20805026 / National Natural Science Foundations of China; National Natural Science Foundation of China (NSFC) 2007CB714505; 2005CB523503 / National Basic Research Program (973 Program) of China; National Basic Research Program of China
- Language
- English
- Date published
- 01/01/2009
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984277266802771
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