Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA

S Perlman; H T Abelson; S Penman

doi:10.1073/pnas.70.2.350

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Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA

Journal article

Open access

Peer reviewed

Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA

S Perlman, H T Abelson and S Penman

Proceedings of the National Academy of Sciences of the United States of America, Vol.70(2), pp.350-353

02/1973

DOI: 10.1073/pnas.70.2.350

PMID: 4510280

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Abstract

A heterogeneous RNA fraction with properties resembling those of messenger RNA was identified in mammalian mitochondria. Synthesis of contaminating RNA of nuclear origin was suppressed by treatment with camptothecin. Labeling of the messenger-like RNA is completely inhibited by ethidium bromide, a specific inhibitor of mitochondrial functions.Although mitochondrial protein synthesis resembles that of prokaryotes in several regards, the messenger-like RNA is covalently linked to poly(adenylic acid) [poly(A)]. Poly(A) has thus far been found only in eukaryotic cells. The poly(A) segment has a gel electrophoretic mobility of about 4 S, corresponding to a length of 50-80 nucleotides, and thus resembles in size the poly(A) found in some mammalian viral RNAs. The messenger RNA can be released from the mitochondrial protein-synthesizing structure by treatment with puromycin.

Cell Fractionation

Electrophoresis, Polyacrylamide Gel

Humans

Carbon Isotopes

RNA, Messenger - analysis

HeLa Cells - metabolism

Tritium

Mitochondria - metabolism

RNA, Messenger - metabolism

Deoxyribonucleases - metabolism

HeLa Cells - drug effects

Mitochondria - analysis

Polynucleotides - analysis

Ribonucleases - metabolism

RNA - biosynthesis

RNA, Messenger - biosynthesis

Adenosine - metabolism

Puromycin - pharmacology

Uridine - metabolism

Adenine - metabolism

Camptothecin - pharmacology

Cell Nucleus

Details

Title: Subtitle: Mitochondrial protein synthesis: RNA with the properties of Eukaryotic messenger RNA
Creators: S Perlman
H T Abelson
S Penman
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences of the United States of America, Vol.70(2), pp.350-353
DOI: 10.1073/pnas.70.2.350
PMID: 4510280
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: United States
Language: English
Date published: 02/1973
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777346302771

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