Journal article
Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?
Aging cell, Vol.15(4), pp.634-645
08/2016
DOI: 10.1111/acel.12472
PMCID: PMC4933659
PMID: 27061426
Abstract
Reactive oxygen species (ROS) are highly reactive oxygen-containing molecules associated with aging and a broad spectrum of pathologies. We have previously shown that transgenic expression of the antioxidant enzyme catalase targeted to the mitochondria (mCAT) in mice reduces ROS, attenuates age-related disease, and increases lifespan. However, it has been increasingly recognized that ROS also has beneficial roles in signaling, hormesis, stress response, and immunity. We therefore hypothesized that mCAT might be beneficial only when ROS approaches pathological levels in older age and might not be advantageous at a younger age when basal ROS is low. We analyzed abundance and turnover of the global proteome in hearts and livers of young (4 month) and old (20 month) mCAT and wild-type (WT) mice. In old hearts and livers of WT mice, protein half-lives were reduced compared to young, while in mCAT mice the reverse was observed; the longest half-lives were seen in old mCAT mice and the shortest in young mCAT. Protein abundance of old mCAT hearts recapitulated a more youthful proteomic expression profile (P-value < 0.01). However, young mCAT mice partially phenocopied the older wild-type proteome (P-value < 0.01). Age strongly interacts with mCAT, consistent with antagonistic pleiotropy in the reverse of the typical direction. These findings underscore the contrasting roles of ROS in young vs. old mice and indicate the need for better understanding of the interaction between dose and age in assessing the efficacy of therapeutic interventions in aging, including mitochondrial antioxidants.
Details
- Title: Subtitle
- Mitochondrial-targeted catalase is good for the old mouse proteome, but not for the young: 'reverse' antagonistic pleiotropy?
- Creators
- Nathan Basisty - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USADao-Fu Dai - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAArni Gagnidze - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USALemuel Gitari - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAJeanne Fredrickson - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAYvonne Maina - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USARichard P Beyer - Department of Environmental Health, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAMary J Emond - Department of Biostatistics, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAEdward J Hsieh - Department of Genome Sciences, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAMichael J MacCoss - Department of Genome Sciences, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAGeorge M Martin - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USAPeter S Rabinovitch - Department of Pathology, University of Washington, 1959 NE Pacific Ave, Seattle, WA, 98195, USA
- Resource Type
- Journal article
- Publication Details
- Aging cell, Vol.15(4), pp.634-645
- DOI
- 10.1111/acel.12472
- PMID
- 27061426
- PMCID
- PMC4933659
- NLM abbreviation
- Aging Cell
- ISSN
- 1474-9718
- eISSN
- 1474-9726
- Publisher
- England
- Grant note
- P41 GM103533 / NIGMS NIH HHS R24 AG042328 / NIA NIH HHS P01 AG001751 / NIA NIH HHS T32 AG000057 / NIA NIH HHS P30 AG013280 / NIA NIH HHS R01 CA210916 / NCI NIH HHS P30 ES007033 / NIEHS NIH HHS
- Language
- English
- Date published
- 08/2016
- Academic Unit
- Pathology; Iowa Neuroscience Institute; Radiation Oncology
- Record Identifier
- 9984046934802771
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