Mitogen-expanded Schwann cells retain the capacity to myelinate regenerating axons after transplantation into rat sciatic nerve

M Laura Feltri; Steven S Scherer; Lawrence Wrabetz; John Kamholz; Michael E Shy

doi:10.1073/pnas.89.18.8827

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Mitogen-expanded Schwann cells retain the capacity to myelinate regenerating axons after transplantation into rat sciatic nerve

Journal article

Open access

Peer reviewed

Mitogen-expanded Schwann cells retain the capacity to myelinate regenerating axons after transplantation into rat sciatic nerve

M Laura Feltri, Steven S Scherer, Lawrence Wrabetz, John Kamholz and Michael E Shy

Proceedings of the National Academy of Sciences - PNAS, Vol.89(18), pp.8827-8831

09/15/1992

DOI: 10.1073/pnas.89.18.8827

PMCID: PMC50014

PMID: 1326765

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Abstract

We have developed a method for genetically modifying Schwann cells (SCs) in vitro and then assessed whether these SCs could interact normally with axons in vivo. Rat SCs were transduced in vitro with the lacZ gene by using a retroviral vector and then expanded with the SC mitogens forskolin and glial growth factor. These mitogen-expanded SCs had an abnormal phenotype as compared to both SCs in vivo and primary SCs in vitro, yet when they were introduced into a regenerating rat sciatic nerve, they formed morphologically normal myelin sheaths around the axons. These results demonstrate that SCs can be genetically altered, their numbers expanded in culture, and yet respond appropriately to axonal signals in the peripheral nervous system. This approach offers a plausible way to manipulate genes involved in axon-SC interactions, including genes that may be defective in some inherited peripheral neuropathies.

Gene Expression

Transfection

Transduction, Genetic

Colforsin - pharmacology

RNA, Messenger - genetics

Rats

Schwann Cells - transplantation

Nerve Regeneration

Microscopy, Electron

Schwann Cells - cytology

Receptors, Nerve Growth Factor

Animals

Glia Maturation Factor

Nerve Tissue Proteins - pharmacology

Sciatic Nerve - cytology

Myelin Sheath - ultrastructure

beta-Galactosidase - genetics

Receptors, Cell Surface - genetics

Details

Title: Subtitle: Mitogen-expanded Schwann cells retain the capacity to myelinate regenerating axons after transplantation into rat sciatic nerve
Creators: M Laura Feltri - Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107
Steven S Scherer
Lawrence Wrabetz
John Kamholz
Michael E Shy
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.89(18), pp.8827-8831
DOI: 10.1073/pnas.89.18.8827
PMID: 1326765
PMCID: PMC50014
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; United States
Grant note: 1-K08NS01261-01A1 / NINDS NIH HHS NS08911-01 / NINDS NIH HHS NS08075 / NINDS NIH HHS
Language: English
Date published: 09/15/1992
Academic Unit: Neurology; Molecular Physiology and Biophysics; Psychiatry; Stead Family Department of Pediatrics; Iowa Neuroscience Institute
Record Identifier: 9984020867902771

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