Journal article
Mitogenomes reveal diversity of the European Lyme borreliosis vector Ixodes ricinus in Italy
Molecular phylogenetics and evolution, Vol.101, pp.194-202
08/2016
DOI: 10.1016/j.ympev.2016.05.009
PMID: 27165938
Abstract
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•We sequenced complete mitogenomes from I. ricinus populations in northern Italy.•Mitogenome phylogenies show 4 I. ricinus lineages that diverged ∼427Kya (95% HPD 330, 544).•Lineage 4 was supported in phylogenies of other short nuclear gene sequences from Europe.•Full mitogenome data provided superior phylogenetic resolution than single nuclear genes.
In Europe, the Ixodes ricinus tick is the most important vector of the etiological agents of Lyme borreliosis and several other emerging tick-borne diseases. Because tick-borne pathogens are dependent on their vectors for transmission, understanding the vector population structure is crucial to inform public health research of pathogen dynamics and spread. However, the population structure and dynamics of this important vector species are not well understood as most genetic studies utilize short mitochondrial and nuclear sequences with little diversity. Herein we obtained and analyzed complete mitochondrial genome (hereafter “mitogenome”) sequences to better understand the genetic diversity and the population structure of I. ricinus from two long-standing tick-borne disease foci in northern Italy. Complete mitogenomes of 23 I. ricinus ticks were sequenced at high coverage. Out of 23 mitogenome sequences we identified 17 unique haplotypes composed of 244 segregating sites. Phylogenetic reconstruction using 18 complete mitogenome sequences revealed the coexistence of four highly divergent I. ricinus maternal lineages despite the narrow spatial scale over which these samples were obtained (100km). Notably, the estimated coalescence time of the 18 mitogenome haplotypes is ∼427 thousand years ago (95% HPD 330, 540). This divergence between I. ricinus lineages is consistent with the mitochondrial diversity of other arthropod vector species and indicates that long-term I. ricinus populations may have been less structured and larger than previously thought. Thus, this study suggests that a rapid and accurate retrieval of full mitochondrial genomes from this disease vector enables fine-resolution studies of tick intraspecies genetic relationships, population differentiation, and demographic history.
Details
- Title: Subtitle
- Mitogenomes reveal diversity of the European Lyme borreliosis vector Ixodes ricinus in Italy
- Creators
- Giovanna Carpi - Pennsylvania State UniversityAndrew Kitchen - University of IowaHie Lim Kim - Nanyang Technological UniversityAakrosh Ratan - University of VirginiaDaniela I Drautz-Moses - Nanyang Technological UniversityJohn J McGraw - Pennsylvania State UniversityMaria Kazimirova - Slovak Academy of SciencesAnnapaola Rizzoli - Fondazione Edmund MachStephan C Schuster - Nanyang Technological University
- Resource Type
- Journal article
- Publication Details
- Molecular phylogenetics and evolution, Vol.101, pp.194-202
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.ympev.2016.05.009
- PMID
- 27165938
- ISSN
- 1055-7903
- eISSN
- 1095-9513
- Grant note
- name: The Autonomous Province of Trento; name: Marie Curie actions-COFUND; name: EU, award: FP7-261504
- Language
- English
- Date published
- 08/2016
- Academic Unit
- Anthropology; International Programs
- Record Identifier
- 9984270193402771
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