Journal article
Mixotrophic cultivation of Galdieria sulphuraria for C-phycocyanin and protein production
Algal research (Amsterdam), Vol.61, p.102603
01/2022
DOI: 10.1016/j.algal.2021.102603
Abstract
G. sulphuraria is a polyextremophilic microalga that can tolerate low pH, high temperature and high osmotic pressure. We cultivated G. sulphuraria ACUF 064 in chemostat at a biomass concentration of 134 to 243 g·m−2 aiming for maximal pigment content without compromising biomass productivity. Autotrophy was compared to ‘oxygen balanced’ mixotrophy with intracellular recirculation of oxygen and carbon dioxide. No differences were found in C-phycocyanin (C-PC) and protein content between autotrophic and mixotrophic cultures. In mixotrophy the biomass productivity and concentration were doubled compared to the photoautotrophic counterpart. In mixotrophy aeration was not needed and 89% of the substrate carbon was converted into biomass.
Mixotrophically grown biomass contained 10% w/w C-PC which, combined with its high areal biomass productivity (49 g·m−2·day−1), sums up as one of the highest C-PC areal productivities ever reported (5 g·m−2·day−1) under 24 h/24 h illumination. C-PC extracted from G. sulphuraria was more stable than the currently used C-PC extracts from Spirulina. No significant loss of color was observed down to a pH of 3 and up to a temperature of 55 °C. G. sulphuraria had a protein content of 62% w/w and compared favorably with FAO dietary recommendation of adults regarding amino acid composition. G. sulphuraria contains a high proportion of essential, sulfur amino acids compared to Chlorella, Spirulina and soybean protein.
Due to its attractive amino acid profile and high protein content, G. sulphuraria is a good candidate for food and feed applications to overcome sulfur amino acid deficiencies. In addition, oxygen balanced mixotrophy allows for efficient and productive cultivation of G. sulphuraria biomass.
•G. sulphuraria was cultivated at biomass concentrations higher than 4 g·L−1.•G. sulphuraria had a high pigment content.•Highest ever reported C-phycocyanin areal productivity in Galdieria with light.•C-phycocyanin showed no significant loss of color down to pH 3 and up to 55 °C.•G. sulphuraria had a high protein and essential amino acid content.
Details
- Title: Subtitle
- Mixotrophic cultivation of Galdieria sulphuraria for C-phycocyanin and protein production
- Creators
- Fabian Abiusi - Wageningen University & ResearchPedro Moñino Fernández - Bioprocess Engineering, AlgaePARC, Wageningen University and Research, P.O. Box 16, 6700 AA Wageningen, the NetherlandsStefano Canziani - Algreen B.V., Bronland 10, 6708 WH Wageningen, the NetherlandsMarcel Janssen - Wageningen University & ResearchRené H. Wijffels - Wageningen University & ResearchMaria Barbosa - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Algal research (Amsterdam), Vol.61, p.102603
- DOI
- 10.1016/j.algal.2021.102603
- ISSN
- 2211-9264
- eISSN
- 2211-9264
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100018693, name: Horizon 2020; DOI: 10.13039/100010661, name: Horizon 2020 Framework Programme, award: 862980
- Language
- English
- Date published
- 01/2022
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984397921602771
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