Journal article
Mn-52 Production for PET/MRI Tracking Of Human Stem Cells Expressing Divalent Metal Transporter 1 (DMT1)
Theranostics, Vol.5(3), pp.227-239
01/01/2015
DOI: 10.7150/thno.10185
PMCID: PMC4279187
PMID: 25553111
Abstract
There is a growing demand for long-term in vivo stem cell imaging for assessing cell therapy techniques and guiding therapeutic decisions. This work develops the production of Mn-52 and establishes proof of concept for the use of divalent metal transporter 1 (DMT1) as a positron emission tomography (PET) and magnetic resonance imaging (MRI) reporter gene for stem cell tracking in the rat brain. Mn-52 was produced via proton irradiation of a natural chromium target. In a comparison of two Mn-52 separation methods, solvent-solvent extraction was preferred over ion exchange chromatography because of reduced chromium impurities and higher Mn-52 recovery. In vitro uptake of Mn-based PET and MRI contrast agents (Mn-52(2+) and Mn2+, respectively) was enhanced in DMT1 over-expressing human neural progenitor cells (hNPC-DMT1) compared to wild-type control cells (hNPC-WT). After cell transplantation in the rat striatum, increased uptake of Mn-based contrast agents in grafted hNPC-DMT1 was detected in in vivo manganese-enhanced MRI (MEMRI) and ex vivo PET and autoradiography. These initial studies indicate that this approach holds promise for dual-modality PET/MR tracking of transplanted stem cells in the central nervous system and prompt further investigation into the clinical applicability of this technique.
Details
- Title: Subtitle
- Mn-52 Production for PET/MRI Tracking Of Human Stem Cells Expressing Divalent Metal Transporter 1 (DMT1)
- Creators
- Christina M. Lewis - University of Wisconsin–MadisonStephen A. Graves - University of Wisconsin–MadisonReinier Hernandez - University of Wisconsin–MadisonHector F. Valdovinos - University of Wisconsin–MadisonTodd E. Barnhart - University of Wisconsin–MadisonWeibo Cai - University of Wisconsin–MadisonMary E. Meyerand - University of Wisconsin–MadisonRobert J. Nickles - University of Wisconsin–MadisonMasatoshi Suzuki - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- Theranostics, Vol.5(3), pp.227-239
- DOI
- 10.7150/thno.10185
- PMID
- 25553111
- PMCID
- PMC4279187
- NLM abbreviation
- Theranostics
- ISSN
- 1838-7640
- eISSN
- 1838-7640
- Publisher
- Ivyspring Int Publ
- Number of pages
- 13
- Grant note
- T32GM008349 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) DGE-1256259 / National Science Foundation; National Science Foundation (NSF) T32CA009206 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) T32 GM008349; NIBIB/NCI 1R01CA169365 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA J10IZ9; 15-IIP-201 / ALS Association DE-SC0008384 / US Department of Energy; United States Department of Energy (DOE)
- Language
- English
- Date published
- 01/01/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Radiation Oncology
- Record Identifier
- 9984383915402771
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