Journal article
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
Journal of Visualized Experiments, Vol.170, e62478
2021
DOI: 10.3791/62478
PMID: 33999028
Abstract
During metastasis, cancer cells from solid tissues, including epithelia, gain access to the lymphatic and hematogenous circulation where they are exposed to mechanical stress due to hemodynamic flow. One of these stresses that circulating tumor cells (CTCs) experience is fluid shear stress (FSS). While cancer cells may experience low levels of FSS within the tumor due to interstitial flow, CTCs are exposed, without extracellular matrix attachment, to much greater levels of FSS. Physiologically, FSS ranges over 3-4 orders of magnitude, with low levels present in lymphatics (<1 dyne/cm2) and the highest levels present briefly as cells pass through the heart and around heart valves (>500 dynes/cm2). There are a few in vitro models designed to model different ranges of physiological shear stress over various time frames. This paper describes a model to investigate the consequences of brief (millisecond) pulses of high-level FSS on cancer cell biology using a simple syringe and needle system.
Details
- Title: Subtitle
- Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
- Creators
- Devon L Moose - University of IowaSophia Williams-Perez - University of IowaRenee Cafun - Roy J. and Lucille A. Carver College of MedicineBenjamin L Krog - University of IowaMichael D Henry - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Journal of Visualized Experiments, Vol.170, e62478
- Publisher
- MyJove Corporation
- DOI
- 10.3791/62478
- PMID
- 33999028
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Language
- English
- Date published
- 2021
- Academic Unit
- Molecular Physiology and Biophysics; Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984302197802771
Metrics
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