Journal article
Models of macromolecular crowding effects and the need for quantitative comparisons with experiment
Current opinion in structural biology, Vol.20(2), pp.196-206
04/2010
DOI: 10.1016/j.sbi.2010.01.008
PMCID: PMC2854290
PMID: 20167475
Abstract
In recent years significant effort has been devoted to exploring the potential effects of macromolecular crowding on protein folding and association phenomena. Theoretical calculations and molecular simulations have, in particular, been exploited to describe aspects of protein behavior in crowded and confined conditions and many aspects of the simulated behavior have reflected, at least at a qualitative level, the behavior observed in experiments. One major and immediate challenge for the theorists is to now produce models capable of making quantitatively accurate predictions of in vitro behavior. A second challenge is to derive models that explain results obtained from experiments performed in vivo, the results of which appear to call into question the assumed dominance of excluded-volume effects in vivo.
Details
- Title: Subtitle
- Models of macromolecular crowding effects and the need for quantitative comparisons with experiment
- Creators
- Adrian H Elcock - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USA. adrian-elcock@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Current opinion in structural biology, Vol.20(2), pp.196-206
- DOI
- 10.1016/j.sbi.2010.01.008
- PMID
- 20167475
- PMCID
- PMC2854290
- NLM abbreviation
- Curr Opin Struct Biol
- ISSN
- 0959-440X
- eISSN
- 1879-033X
- Publisher
- England
- Grant note
- R01 GM087290-01 / NIGMS NIH HHS R01 GM087290 / NIGMS NIH HHS GM087290 / NIGMS NIH HHS
- Language
- English
- Date published
- 04/2010
- Academic Unit
- Physics and Astronomy; Biochemistry and Molecular Biology
- Record Identifier
- 9984025255602771
Metrics
18 Record Views