Journal article
Modification of surface properties of oral streptococci by α-1,6 glucans
Colloids and surfaces, B, Biointerfaces, Vol.8(6), pp.295-302
1997
DOI: 10.1016/S0927-7765(96)01328-8
Abstract
Several members of the genus
Streptococcus, including
S. sobrinus, S. cricetus, S. sanguis and
S. mutans, are known to produce proteins capable of complexing with α-1,6 glucans. Of all the oral streptococcal groups,
S. sobrinus and
S. cricetus are the most readily aggregated by high molecular weight glucan (glucan T-2000), but the aggregation may be inhibited by low molecular weight glucans (glucan T-10). Some of the oral streptococci are also known to express surface hydrophobins, molecules which impart hydrophobic characteristics to their cell surfaces. When glucan T-10 was added to suspensions of
S. cricetus or
S. sobrinus, the bacteria were less able to adhere to hexadecane, to bind to plastics or to be aggregated by ammonium sulfate. It is concluded that the glucan rendered the bacteria more hydrophilic. In contrast, streptococci such as
S. sanguis and
S. mutans, incapable of aggregation by high molecular weight α-1,6 glucan, retained their hydrophobic characteristics in the presence of glucan T-10. Other bacteria, such as
Bacillus subtilis, Escherichia coli and
Pseudomonas aeruginosa, exhibited their same abilities to bind to hexadecane, to adhere to plastics or to be aggregated by ammonium sulfate, regardless of the presence of α-1,6 glucan. When
S. cricetus or
S. sobrinus were grown under conditions to minimize expression of glucan-binding lectin or when glucan-binding lectin was denatured, glucan T-10 had no effect on their hydrophobic characteristics. It appears that the complexing of α-1,6 glucan by streptococci bearing glucan-binding lectin causes the bacteria to assume a more hydrophilic character.
Details
- Title: Subtitle
- Modification of surface properties of oral streptococci by α-1,6 glucans
- Creators
- Lodi G. Mata - University of LouisvilleD. Drake - University of IowaR.J. Doyle - University of Louisville
- Resource Type
- Journal article
- Publication Details
- Colloids and surfaces, B, Biointerfaces, Vol.8(6), pp.295-302
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0927-7765(96)01328-8
- ISSN
- 0927-7765
- eISSN
- 1873-4367
- Language
- English
- Date published
- 1997
- Academic Unit
- Endodontics; Dental Research
- Record Identifier
- 9984367655302771
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