Journal article
Modulating numbers and phenotype of CD8+ T cells in secondary immune responses
European journal of immunology, Vol.40(7), pp.1916-1926
07/2010
DOI: 10.1002/eji.201040310
PMCID: PMC2993099
PMID: 20411564
Abstract
Prime-boost regimens are frequently used to increase the number of memory CD8
+
T cells and thus the protective capacity of experimental vaccinations; however, it is currently unknown how the frequency and phenotype of primary (1°) memory CD8
+
T cells impact the quantity and phenotype of secondary (2°) memory CD8
+
T-cell populations. Here, we show that 2° infections of mice that received different 1° infections and/or immunizations generated similar numbers of 2° effector and memory CD8
+
T cells. Remarkably, this result was independent of the numbers and phenotype of 1° memory CD8
+
T cells present at the time of rechallenge. However, after adoptive transfer of low numbers of 1° memory CD8
+
T cells, a linear correlation between 1° memory CD8
+
T-cell input and 2° memory CD8
+
T-cell numbers was observed. These data suggest that, above a very low threshold, boosting of 1° memory CD8
+
T-cell populations elicits 2° immune responses of similar magnitude. Therefore, our study has important implications for the design of prime-boost regimens that aim to generate protective CD8
+
T-cell-mediated immunity.
Details
- Title: Subtitle
- Modulating numbers and phenotype of CD8+ T cells in secondary immune responses
- Creators
- Thomas C Wirth - Department of Microbiology, University of Iowa, Iowa City, IA, USAJohn T Harty - Department of Microbiology, University of Iowa, Iowa City, IA, USAVladimir P Badovinac - Interdisciplinary Graduate Program in Immunology, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- European journal of immunology, Vol.40(7), pp.1916-1926
- DOI
- 10.1002/eji.201040310
- PMID
- 20411564
- PMCID
- PMC2993099
- ISSN
- 0014-2980
- eISSN
- 1521-4141
- Language
- English
- Date published
- 07/2010
- Academic Unit
- Microbiology and Immunology; Pathology
- Record Identifier
- 9984046804502771
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