Journal article
Modulation of Amniotic Fluid Activin-A and Inhibin-A in Women With Preterm Premature Rupture of the Membranes and Infection-Induced Preterm Birth
American journal of reproductive immunology (1989), Vol.67(2), pp.122-131
02/2012
DOI: 10.1111/j.1600-0897.2011.01074.x
PMID: 21992678
Abstract
Problem
Activins and inhibins are important modulators of inflammatory processes. We explored activation of amniotic fluid (AF) activin-A and inhibin-A system in women with intra-amniotic infection and preterm premature rupture of the membranes (PPROM).
Method of study
We analyzed 78 AF samples: '2nd trimester-control' (n = 12), '3rd trimester-control' (n = 14), preterm labor with intact membranes [positive-AF-cultures (n = 13), negative-AF-cultures (n = 13)], and PPROM [positive-AF-cultures (n = 13), negative-AF-cultures (n = 13)]. Activin-A levels were evaluated ex-vivo following incubation of amniochorion and placental villous explants with Gram-negative lipopolysaccharide (LPS) or Gram-positive (Pam3Cys) bacterial mimics. Ability of recombinant activin-A and inhibin-A to modulate inflammatory reactions in fetal membranes was explored through explants' IL-8 release.
Results
Activin-A and inhibin-A were present in human AF and were gestational age-regulated. Activin-A was significantly upregulated by infection. Lower inhibin-A levels were seen in PPROM. LPS elicited release of activin-A from amniochorion, but not from villous explants. Recombinant activin-A stimulated IL-8 release from amniochorion, an effect that was not reversed by inhibin-A.
Conclusion
Human AF activin-A and inhibin-A are involved in biological processes linked to intra-amniotic infection / inflammation-induced preterm birth.
Details
- Title: Subtitle
- Modulation of Amniotic Fluid Activin-A and Inhibin-A in Women With Preterm Premature Rupture of the Membranes and Infection-Induced Preterm Birth
- Creators
- Victor A. Rosenberg - Yale UniversityIrina A. Buhimschi - Yale UniversityAntonette T. Dulay - Yale UniversitySonya S. Abdel-Razeq - Yale UniversityEmily A. Oliver - Queen Mary University of LondonChristina M. Duzyj - Yale UniversityHeather Lipkind - Yale UniversityChristian M. Pettker - Yale UniversityCatalin S. Buhimschi - Yale University
- Resource Type
- Journal article
- Publication Details
- American journal of reproductive immunology (1989), Vol.67(2), pp.122-131
- DOI
- 10.1111/j.1600-0897.2011.01074.x
- PMID
- 21992678
- NLM abbreviation
- Am J Reprod Immunol
- ISSN
- 1046-7408
- eISSN
- 1600-0897
- Publisher
- Wiley
- Number of pages
- 10
- Grant note
- Yale University Department of Obstetrics, Gynecology and Reproductive Sciences Department of Pediatrics, Division of Perinatal Medicine at Yale New Haven Hospital RO1 HD 047321 / National Institute of Health (NIH)/Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) Department of Obstetrics, Gynecology and Reproductive Sciences R01HD062007 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) R01HD047321 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Language
- English
- Date published
- 02/2012
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9985244663202771
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