Journal article
Modulation of Wnt/[beta]-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells
Brazilian journal of medical and biological research, Vol.53(8), e9488
08/01/2020
DOI: 10.1590/1414-431X20209488
PMCID: PMC7307890
PMID: 32578719
Abstract
Macrophages play pivotal roles in host defense and immune homeostasis, which have two major functional polarization states, the classically activated M1 and the alternatively activated M2. Interleukin (IL)-17A is an immune modulator able to shape macrophage phenotypes. Wnt/[beta]-catenin is a developmental signaling pathway that plays crucial roles in morphogenesis and tissue homeostasis, which has also been recently demonstrated playing roles in immune regulation. A growing amount of evidence suggests that both Wnt and IL-17A signaling are involved in macrophage polarization. However, their interaction in macrophage polarization remains elusive. The aim of present study was to explore impacts of Wnt/[beta]-catenin on IL-17A-mediated macrophage M1/M2 polarization in murine monocyte/macrophage-like cell line RAW264.7. Results revealed that IL17A activated Wnt/[beta]-catenin signaling and induced macrophage M1 polarization, but inhibited M2 polarization. In contrast, the activation of Wnt/[beta]-catenin signaling led to the inhibition of M1 macrophage polarization but the promotion of M2 polarization. Importantly, the activation of Wnt/[beta]-catenin also showed abilities to inhibit the IL-17A-induced M1 macrophage polarization while diminishing the IL-17A-inhibited M2 polarization. Molecular analysis further uncovered that the JAK/STATsignaling pathway was involved in the interaction of Wnt/[beta]-catenin and IL-17A in the modulation of macrophage polarization. These results suggested that the Wnt/[beta]-catenin signaling modulated IL-17A-altered macrophage polarization in part by regulating the JAK/STAT signaling pathway. This study thus revealed a novel function of Wnt/[beta]-catenin signaling in regulating IL-17A-altered macrophage polarization. Keywords: Wnt/[beta]-catenin signaling; Interleukin-17A; Macrophage polarization; STAT; Immune regulation
Details
- Title: Subtitle
- Modulation of Wnt/[beta]-catenin signaling in IL-17A-mediated macrophage polarization of RAW264.7 cells
- Creators
- Jing Xue - Ningxia UniversityJia Ma - Ningxia UniversityChao Yuan - Ningxia UniversityJiali Yang - Ningxia UniversityFuyang Song - Ningxia UniversityXiaoming Liu - Ningxia UniversityDandan Yang - Ningxia University
- Resource Type
- Journal article
- Publication Details
- Brazilian journal of medical and biological research, Vol.53(8), e9488
- DOI
- 10.1590/1414-431X20209488
- PMID
- 32578719
- PMCID
- PMC7307890
- NLM abbreviation
- Braz J Med Biol Res
- ISSN
- 0100-879X
- eISSN
- 1414-431X
- Publisher
- Associacao Brasileira de Divulgacao Cientifica (ABDC)
- Language
- English
- Date published
- 08/01/2020
- Description audience
- Academic
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984288748702771
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