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Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A
Journal article   Open access   Peer reviewed

Modulation of embryonic intracellular Ca2+ signaling by Wnt-5A

D C Slusarski, J Yang-Snyder, W B Busa and R T Moon
Developmental biology, Vol.182(1), pp.114-120
02/01/1997
DOI: 10.1006/dbio.1996.8463
PMID: 9073455
url
https://doi.org/10.1006/dbio.1996.8463View
Published (Version of record) Open Access

Abstract

Wnt genes encode secreted proteins which are implicated in receptor-mediated cell-cell signaling events important in embryogenesis, but the second messenger systems modulated by Wnts have not been identified. We report that ectopic expression of Xwnt-5A in zebrafish embryos enhances the frequency of intracellular Ca2+ transients in the enveloping layer of the blastodisc, whereas Xwnt-8 does not. These transients are independent of extracellular Ca2+. Consistent with the observed Ca2+ transients playing a role in responses of embryos to Xwnt-5A, we report that the ligand-activated serotonin type 1C receptor, which stimulates PI cycle activity and Ca2+ signaling independent of Wnts, phenocopies embryonic responses to Xwnt-5A. These results suggest that intercellular signaling by a subset of vertebrate Wnts involves modulation of a intracellular Ca2+ signaling pathway, which may arise from phosphatidylinositol cycle activity.
Receptors, Serotonin - physiology Receptors, Serotonin - biosynthesis Signal Transduction Calcium - metabolism Blastocyst - physiology Zebrafish Wnt Proteins Wnt-5a Protein Proto-Oncogene Proteins - biosynthesis Receptor, Serotonin, 5-HT2C Vertebrates Animals Proto-Oncogene Proteins - physiology Embryo, Nonmammalian - physiology

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