Journal article
Modulation of experimental autoimmune encephalomyelitis: effect of altered peptide ligand on chemokine and chemokine receptor expression
Journal of neuroimmunology, Vol.110(1), pp.195-208
2000
DOI: 10.1016/S0165-5728(00)00351-9
PMID: 11024550
Abstract
Experimental autoimmune encephalomyelitis (EAE) is a T helper 1 (Th1) cell mediated demyelinating disease and the principal animal model for multiple sclerosis. Spinal cords from SJL mice primed with proteolipid protein peptide 139-151 (pPLP) expressed the chemokines RANTES, MCP-1, MIP-2, KC, MIP-1α, MIP-1β, Mig, and fractalkine. We also identified IP-10 in these samples and described a sequence polymorphism in this transcript. Chemokine expression was specific for tissues of the central nervous system. MCP-1, IP-10, and MIP-2 RNA expression significantly correlated with clinical score. Chemokine receptor expression generally correlated with ligand expression. pPLP-primed mice expressed the Th1-associated markers CCR5 and CXCR3 on mononuclear cells. In addition, cells expressing CCR1, CCR2, CCR3, CCR4, CCR8, and CXCR2 were detected. Here we demonstrate that altered peptide ligand (APL)-induced protection from EAE was accompanied by modulation of chemokine and chemokine receptor expression. Spinal cord tissue sections from APL-protected mice showed greatly reduced levels of all chemokines and of CCR1, CCR5, CCR8, CXCR2 and CXCR3. The Th2-associated chemokine receptors CCR3 and CCR4 were found in protected mice, supporting the hypothesis that Th1 but not Th2 cells are down-regulated by APL treatment. This report concludes that chemokines and chemokine receptors can be useful tools to follow modulation of autoimmune disease.
Details
- Title: Subtitle
- Modulation of experimental autoimmune encephalomyelitis: effect of altered peptide ligand on chemokine and chemokine receptor expression
- Creators
- Falko R Fischer - Harvard UniversityLaura Santambrogio - Harvard UniversityYi Luo - Harvard UniversityMichael A Berman - Harvard UniversityWayne W Hancock - Millennium Engineering and IntegrationMartin E Dorf - Harvard University
- Resource Type
- Journal article
- Publication Details
- Journal of neuroimmunology, Vol.110(1), pp.195-208
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0165-5728(00)00351-9
- PMID
- 11024550
- ISSN
- 0165-5728
- eISSN
- 1872-8421
- Language
- English
- Date published
- 2000
- Academic Unit
- Urology
- Record Identifier
- 9984320069402771
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