Journal article
Modulation of glucan-binding protein activity in streptococci by fluoride
Biochimica et biophysica acta. General subjects, Vol.1474(3), pp.346-352
2000
DOI: 10.1016/S0304-4165(00)00024-6
PMID: 10779686
Abstract
Glucan-binding lectin (GBL) activity of
Streptococcus sobrinus was significantly reduced by fluoride in the growth medium. Approximately 1.5 mM fluoride was required for a 50% reduction in GBL activity. In addition to the GBL, several other glucan-binding proteins were reduced when the bacteria were grown in subinhibitory fluoride. Fluoride had no effect on glucosyltransferases (GTFs), enzymes capable of converting sucrose into α-1,6-glucans. All the proteins were detected by use of enhanced chemiluminescence (ECL of fluorescein-labeled dextran) and Western blotting of renatured SDS–PAGE gels. The effects of fluoride on the bacteria were abrogated when the manganous ion was included in the growth medium. It thus appears that one mechanism of action of fluoridated water is its effects on glucan-binding proteins. The fluoride may be reducing metabolism of the mangano aquo ion, essential for expression of the glucan-binding proteins.
Details
- Title: Subtitle
- Modulation of glucan-binding protein activity in streptococci by fluoride
- Creators
- Somkiat Luengpailin - Department of Microbiology and Immunology, University of Louisville, Health Science Center, Louisville, KY40292, USAJ.A Banas - Department of Microbiology, Immunology, and Molecular Genetics, Albany Medical College, Albany, NY 12208, USAR.J Doyle - Department of Microbiology and Immunology, University of Louisville, Health Science Center, Louisville, KY40292, USA
- Resource Type
- Journal article
- Publication Details
- Biochimica et biophysica acta. General subjects, Vol.1474(3), pp.346-352
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0304-4165(00)00024-6
- PMID
- 10779686
- ISSN
- 0304-4165
- eISSN
- 1872-8006
- Language
- English
- Date published
- 2000
- Academic Unit
- Pediatric Dentistry; Dental Research
- Record Identifier
- 9984066093202771
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