Journal article
Modulation of murine dendritic cell function by adenine nucleotides and adenosine: Involvement of the A(2B) receptor
European journal of immunology, Vol.38(6), pp.1610-1620
06/01/2008
DOI: 10.1002/eji.200737781
PMID: 18465770
Abstract
Adenosine triphosphate has previously been shown to induce semi-mature human monocyte-derived dendritic cells (DC). These are characterized by the up-regulation of co-stimulatory molecules, the inhibition of IL-12 and the up-regulation of some genes involved in immune tolerance, such as thrombospondin-1 and indoleamine 2,3-dioxygenase. The actions of adenosine triphosphate are mediated by the P2Y(11) receptor; since there is no functional P2Y(11) gene in the murine genome, we investigated the action of adenine nucleotides on murine DC. Adenosine 5'-(3-thiotriphosphate) and adenosine inhibited the production of IL-12p70 by bone marrow-derived DC (BMDC). These inhibitions were relieved by 8-p-sulfophenyltheophylline, an adenosine receptor antagonist. The use of selective ligands and A(2B)(-/-) BMDC indicated the involvement of the A(2B) receptor. A microarray experiment, confirmed by quantitative PCR, showed that, in presence of LPS, 5'-(N-ethylcarboxamido) adenosine (NECA, the most potent A(2B) receptor agonist) regulated the expression of several genes: arginase I and II, thrombospondin-1 and vascular endothelial growth factor were up-regulated whereas CCL2 and CCL12 were down-regulated. We further showed that NECA, in combination with LPS, increased the arginase I enzymatic activity. In conclusion, the described actions of adenine nucleotides on BMDC are mediated. by their degradation product, adenosine, acting on the A(2B) receptor, and will possibly lead to an impairment of Th1 response or tolerance.
Details
- Title: Subtitle
- Modulation of murine dendritic cell function by adenine nucleotides and adenosine: Involvement of the A(2B) receptor
- Creators
- Abduelhakem Ben Addi - Université Libre de BruxellesAnne Lefort - Université Libre de BruxellesXiaoyang Hua - University of North Carolina at Chapel HillFrederick Libert - Université Libre de BruxellesDidier Communi - Université Libre de BruxellesCatherine Ledent - Université Libre de BruxellesPascale Macours - Université Libre de BruxellesStephen L. Tilley - University of North Carolina at Chapel HillJean-Marie Boeynaems - Université Libre de BruxellesBernard Robaye - Université Libre de Bruxelles
- Resource Type
- Journal article
- Publication Details
- European journal of immunology, Vol.38(6), pp.1610-1620
- Publisher
- Wiley
- DOI
- 10.1002/eji.200737781
- PMID
- 18465770
- ISSN
- 0014-2980
- eISSN
- 1521-4141
- Number of pages
- 11
- Language
- English
- Date published
- 06/01/2008
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984312244502771
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