Journal article
Modulation of the Immunological Milieu in Acute Aneurysmal Subarachnoid Hemorrhage: The Potential Role of Monocytes Through CXCL10 Secretion
Translational stroke research, Vol.16(1), pp.88-95
02/2025
DOI: 10.1007/s12975-024-01259-4
PMID: 38780865
Abstract
Emerging evidence indicates that aneurysmal subarachnoid hemorrhage (aSAH) elicits a response from both innate and adaptive immune systems. An upregulation of CD8 + CD161 + cells has been observed in the cerebrospinal fluid (CSF) after aSAH, yet the precise role of these cells in the context of aSAH is unkown. CSF samples from patients with aSAH and non-aneurysmal SAH (naSAH) were analyzed. Single-cell RNA sequencing (scRNAseq) was performed on CD8 + CD161 + sorted samples from aSAH patients. Cell populations were identified using "clustering." Gene expression levels of ten previously described genes involved in inflammation were quantified from aSAH and naSAH samples using RT-qPCR. The study focused on the following genes: CCL5, CCL7, APOE, SPP1, CXCL8, CXCL10, HMOX1, LTB, MAL, and HLA-DRB1. Gene clustering analysis revealed that monocytes, NK cells, and T cells expressed CD8 + CD161 + in the CSF of patients with aSAH. In comparison to naSAH samples, aSAH samples exhibited higher mRNA levels of CXCL10 (median, IQR = 90, 16-149 vs. 0.5, 0-6.75, p = 0.02). A trend towards higher HMOX1 levels was also observed in aSAH (median, IQR = 12.6, 9-17.6 vs. 2.55, 1.68-5.7, p = 0.076). Specifically, CXCL10 and HMOX1 were expressed by the monocyte subpopulation. Monocytes, NK cells, and T cells can potentially express CD8 + CD161 + in patients with aSAH. Notably, monocytes show high levels of CXCL10. The elevated expression of CXCL10 in aSAH compared to naSAH indicates its potential significance as a target for future studies.
Details
- Title: Subtitle
- Modulation of the Immunological Milieu in Acute Aneurysmal Subarachnoid Hemorrhage: The Potential Role of Monocytes Through CXCL10 Secretion
- Creators
- Sebastian Sanchez - Yale UniversityMichael S Chimenti - University of IowaYongjun Lu - University of IowaElena Sagues - University of IowaAndres Gudino - University of IowaCarlos Dier - University of IowaDavid Hasan - Duke UniversityEdgar A Samaniego - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Translational stroke research, Vol.16(1), pp.88-95
- DOI
- 10.1007/s12975-024-01259-4
- PMID
- 38780865
- eISSN
- 1868-601X
- Language
- English
- Electronic publication date
- 05/23/2024
- Date published
- 02/2025
- Academic Unit
- Neurology; Radiology; Critical Care; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neurosurgery; Iowa Institute of Human Genetics
- Record Identifier
- 9984628216702771
Metrics
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