Journal article
Modulation of the frequency response of Shaker potassium channels by the quiver peptide suggesting a novel extracellular interaction mechanism
Journal of neurogenetics, Vol.24(2), pp.67-74
07/2010
DOI: 10.3109/01677061003746341
PMCID: PMC3115930
PMID: 20429677
Abstract
Recent studies have indicated that the Shaker potassium channel regulates sleep in Drosophila. The Drosophila quiver (qvr) gene encodes a novel potassium channel subunit that modulates the Shaker potassium channel. The Qvr peptide contains a signal sequence for extracellular localization and may regulate a unique feature of the Shaker I(A) current that confers special neuronal excitability patterns. Thus, studies of the Shaker channel properties in the qvr mutant background should provide an opportunity to uncover a new form of physiologic modulation of potassium channels. We have begun to investigate the impact of qvr protein on the Shaker channel properties and its implications in synaptic function in vivo. We studied synaptic transmission at the larval neuromuscular junction and characterized the transient potassium current I(A) in larval muscles. We identified two different functional states of I(A) in qvr larval muscles, as reflected by two distinct components, I(AF) and I(AS), differing in their kinetics of recovery from inactivation and sensitivity to a K(+) channel blocker. Correspondingly, qvr mutant larvae exhibit multiple synaptic discharges following individual nerve stimuli during repetitive activity.
Details
- Title: Subtitle
- Modulation of the frequency response of Shaker potassium channels by the quiver peptide suggesting a novel extracellular interaction mechanism
- Creators
- Jing W Wang - Department of Biological Sciences, University of Iowa, Iowa City, Iowa, USA. jw800@ucsd.eduChun-Fang Wu
- Resource Type
- Journal article
- Publication Details
- Journal of neurogenetics, Vol.24(2), pp.67-74
- DOI
- 10.3109/01677061003746341
- PMID
- 20429677
- PMCID
- PMC3115930
- NLM abbreviation
- J Neurogenet
- ISSN
- 0167-7063
- eISSN
- 1563-5260
- Publisher
- England
- Grant note
- R01 NS026528 / NINDS NIH HHS\nNS026528 / NINDS NIH HHS\nGM088804 / NIGMS NIH HHS\nR01 GM088804 / NIGMS NIH HHS\nR01 NS026528-12 / NINDS NIH HHS\nR01 GM088804-20 / NIGMS NIH HHS
- Language
- English
- Date published
- 07/2010
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070212302771
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