Journal article
Modulation of the lung host response by granulocyte colony-stimulating factor in rats challenged with intrapulmonary endotoxin
Shock (Augusta, Ga.), Vol.7(3), pp.193-199
03/1997
DOI: 10.1097/00024382-199703000-00007
PMID: 9068085
Abstract
The effects of granulocyte colony-stimulating factor (G-CSF) on the functional activities of circulating and lung-recruited neutrophils (PMNs) and alveolar macrophages (AMs) were studied in rats to further elucidate the mechanisms underlying G-CSF-enhanced pulmonary host defense. Animals received G-CSF or vehicle twice a day for 2 days, followed by an intratracheal challenge with endotoxin or saline. G-CSF up-regulated CD11b/c expression and mean channel fluorescence intensity of phagocytosis in circulating PMNs. G-CSF also enhanced phagocytic activities, reflected by both the percentage of phagocytosis and mean channel fluorescence intensity in lung-recruited PMNs and AMs in intratracheal endotoxin-challenged rats. The endotoxin-induced increase in pulmonary production of tumor necrosis factor-alpha and cytokine-induced neutrophil chemoattractant was not affected by G-CSF pretreatment. These data demonstrate that G-CSF-enhanced pulmonary recruitment of PMNs is primarily based on the effects of G-CSF on the PMNs themselves, rather than the generation of certain chemotactic stimuli, i.e., cytokine-induced neutrophil chemoattractant and tumor necrosis factor-alpha. The enhanced phagocytic activities of lung-recruited PMNs and AMs also augment pulmonary host defenses in G-CSF-pretreated animals.
Details
- Title: Subtitle
- Modulation of the lung host response by granulocyte colony-stimulating factor in rats challenged with intrapulmonary endotoxin
- Creators
- Ping Zhang - Department of Medicine, Louisiana State University Medical Center, New Orleans 70112, USAGregory J BagbyD A StoltzJudy A SpitzerWarren R SummerSteve Nelson
- Resource Type
- Journal article
- Publication Details
- Shock (Augusta, Ga.), Vol.7(3), pp.193-199
- Publisher
- United States
- DOI
- 10.1097/00024382-199703000-00007
- PMID
- 9068085
- ISSN
- 1073-2322
- eISSN
- 1540-0514
- Grant note
- AA-09803 / NIAAA NIH HHS
- Language
- English
- Date published
- 03/1997
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984025577502771
Metrics
22 Record Views