Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein

Sandhya Shankarnarayan; Sandhya S Narang; Cheryl L Malone; Robert J Deschenes; Jan S Fassler

doi:10.1074/jbc.M706877200

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Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein

Journal article

Open access

Peer reviewed

Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein

Sandhya Shankarnarayan, Sandhya S Narang, Cheryl L Malone, Robert J Deschenes and Jan S Fassler

The Journal of biological chemistry, Vol.283(4), pp.1962-1973

01/25/2008

DOI: 10.1074/jbc.M706877200

PMCID: PMC2892218

PMID: 18048366

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Abstract

The yeast Sln1p sensor kinase is best known as an osmosensor involved in the regulation of the hyperosmolarity glycerol mitogen-activated protein kinase cascade. Down-regulation of Sln1 kinase activity occurs under hypertonic conditions and leads to phosphorylation of the Hog1p mitogen-activated protein kinase and increased osmotic stress-response gene expression. Conditions leading to kinase up-regulation include osmotic imbalance caused by glycerol retention in the glycerol channel mutant, fps1 (Tao, W., Deschenes, R. J., and Fassler, J. S. (1999) J. Biol. Chem. 274, 360-367). The hypothesis that Sln1p kinase activity is responsive to turgor was first suggested by the increased Sln1p kinase activity in mutants lacking Fps1p in which glycerol accumulation leads to water uptake. Also consistent with the turgor hypothesis is the observation that reduced turgor caused by treatment of cells with nystatin, a drug that increases membrane permeability and causes cell shrinkage, reduced Sln1p kinase activity (Tao, W., Deschenes, R. J., and Fassler, J. S. (1999) J. Biol. Chem. 274, 360-367; Reiser, V., Raitt, D. C., and Saito, H. (2003) J. Cell Biol. 161, 1035-1040). The turgor hypothesis is revisited here in the context of the identification and characterization of the cell wall gene, CCW12, as a determinant of Sln1p activity. Results of this analysis suggest that the activity of the plasma membrane localized Sln1p is affected by the presence or absence of specific outer cell wall proteins and that this effect is independent of turgor.

Antifungal Agents - pharmacology

Cell Membrane Permeability - drug effects

Cell Membrane Permeability - physiology

Cell Wall - genetics

Cell Wall - metabolism

Glycerol - metabolism

Glycoproteins

Intracellular Signaling Peptides and Proteins

MAP Kinase Signaling System - drug effects

MAP Kinase Signaling System - physiology

Mitogen-Activated Protein Kinases - genetics

Mitogen-Activated Protein Kinases - metabolism

Nystatin - pharmacology

Osmosis - physiology

Protein Kinases - genetics

Protein Kinases - metabolism

Saccharomyces cerevisiae - cytology

Saccharomyces cerevisiae - enzymology

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae Proteins - genetics

Saccharomyces cerevisiae Proteins - metabolism

Water-Electrolyte Balance - physiology

Details

Title: Subtitle: Modulation of yeast Sln1 kinase activity by the CCW12 cell wall protein
Creators: Sandhya Shankarnarayan - Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA
Sandhya S Narang
Cheryl L Malone
Robert J Deschenes
Jan S Fassler
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.283(4), pp.1962-1973
DOI: 10.1074/jbc.M706877200
PMID: 18048366
PMCID: PMC2892218
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Grant note: R01 GM056719 / NIGMS NIH HHS R01 GM056719-08 / NIGMS NIH HHS GM56719 / NIGMS NIH HHS
Language: English
Date published: 01/25/2008
Academic Unit: Biology
Record Identifier: 9984217416802771

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