Journal article
Molecular Analyses of an Unusual Translesion DNA Polymerase from Methanosarcina acetivorans C2A
Journal of molecular biology, Vol.397(1), pp.13-30
2010
DOI: 10.1016/j.jmb.2010.01.007
PMID: 20080107
Abstract
The domain Archaea is composed of several subdomains, and prominent among them are the Crenarchaeota and the Euryarchaeota. Biochemically characterized archaeal family Y DNA polymerases (Pols) or DinB homologs, to date, are all from crenarchaeal organisms, especially the genus
Sulfolobus. Here, we demonstrate that archaeal family Y Pols fall into five clusters based on phylogenetic analysis. MacDinB-1, the homolog from the euryarchaeon
Methanosarcina acetivorans that is characterized in this study, belongs to cluster II. Therefore, MacDinB-1 is different from the
Sulfolobus DinB proteins, which are members of cluster I. In addition to translesion DNA synthesis activity, MacDinB-1 synthesized unusually long products (∼
7.2 kb) in the presence of its cognate proliferating cell nuclear antigen (PCNA). The PCNA-interacting site in MacDinB-1 was identified by mutational analysis in a C-terminally located heptapeptide akin to a PIP (PCNA-interacting protein) box.
In vitro assays from the present report suggested that MacDinB-1 works in an error-free mode to repair cyclobutane pyrimidine dimers. This study on a euryarchaeal DinB homolog provides important insights into the functional diversity of the family Y Pols, and the availability of a genetic system for this archaeon should allow subsequent elucidation of the physiological significance of this enzyme in
M. acetivorans cells.
Details
- Title: Subtitle
- Molecular Analyses of an Unusual Translesion DNA Polymerase from Methanosarcina acetivorans C2A
- Creators
- Li-Jung Lin - University of Illinois Urbana-ChampaignAya Yoshinaga - Kyushu UniversityYuyen Lin - University of Illinois Urbana-ChampaignClaudia Guzman - University of Illinois Urbana-ChampaignYi-Hsing Chen - University of Illinois Urbana-ChampaignShou Mei - University of Illinois Urbana-ChampaignAngelica M. Lagunas - University of Illinois Urbana-ChampaignSatoshi Koike - Hokkaido UniversityShigenori Iwai - Osaka UniversityM. Ashley Spies - University of Illinois Urbana-ChampaignSatish K. Nair - University of Illinois Urbana-ChampaignRoderick I. Mackie - University of Illinois Urbana-ChampaignYoshizumi Ishino - Kyushu UniversityIsaac K.O. Cann - University of Illinois Urbana-Champaign
- Resource Type
- Journal article
- Publication Details
- Journal of molecular biology, Vol.397(1), pp.13-30
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/j.jmb.2010.01.007
- PMID
- 20080107
- ISSN
- 0022-2836
- eISSN
- 1089-8638
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation, award: MCB 0238451
- Language
- English
- Date published
- 2010
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984293087702771
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