Journal article
Molecular Ontology of the Nucleus of Solitary Tract
Journal of comparative neurology (1911), Vol.532(12), e70004
12/01/2024
DOI: 10.1002/cne.70004
PMCID: PMC11615840
PMID: 39629676
Appears in UI Libraries Support Open Access
Abstract
The nucleus of the solitary tract (NTS) receives visceral information and regulates appetitive, digestive, and cardiorespiratory systems. Within the NTS, diverse processes operate in parallel to sustain life, but our understanding of their cellular composition is incomplete. Here, we integrate histologic and transcriptomic analysis to identify and compare molecular features that distinguish neurons in this brain region. Most glutamatergic neurons in the NTS and area postrema co-express the transcription factors Lmx1b and Phox2b, except for a ventral band of neurons in the far-caudal NTS, which include the Gcg-expressing neurons that produce glucagon-like peptide 1 (GLP-1). GABAergic interneurons intermingle through the Lmx1b+Phox2b macropopulation, and dense clusters of GABAergic neurons surround the NTS. The Lmx1b+Phox2b macropopulation includes subpopulations with distinct distributions expressing Grp, Hsd11b2, Npff, Pdyn, Pou3f1, Sctr, Th, and other markers. These findings highlight Lmx1b-Phox2b co-expression as a common feature of glutamatergic neurons in the NTS and improve our understanding of the organization and distribution of neurons in this critical brain region.The nucleus of the solitary tract (NTS) receives visceral information and regulates appetitive, digestive, and cardiorespiratory systems. Within the NTS, diverse processes operate in parallel to sustain life, but our understanding of their cellular composition is incomplete. Here, we integrate histologic and transcriptomic analysis to identify and compare molecular features that distinguish neurons in this brain region. Most glutamatergic neurons in the NTS and area postrema co-express the transcription factors Lmx1b and Phox2b, except for a ventral band of neurons in the far-caudal NTS, which include the Gcg-expressing neurons that produce glucagon-like peptide 1 (GLP-1). GABAergic interneurons intermingle through the Lmx1b+Phox2b macropopulation, and dense clusters of GABAergic neurons surround the NTS. The Lmx1b+Phox2b macropopulation includes subpopulations with distinct distributions expressing Grp, Hsd11b2, Npff, Pdyn, Pou3f1, Sctr, Th, and other markers. These findings highlight Lmx1b-Phox2b co-expression as a common feature of glutamatergic neurons in the NTS and improve our understanding of the organization and distribution of neurons in this critical brain region.
Details
- Title: Subtitle
- Molecular Ontology of the Nucleus of Solitary Tract
- Creators
- Silvia Gasparini - University of IowaGislaine Almeida-Pereira - University of Iowa, NeurologyAna Sofia Peraza Munuzuri - University of IowaJon M Resch - University of IowaJoel C Geerling - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of comparative neurology (1911), Vol.532(12), e70004
- DOI
- 10.1002/cne.70004
- PMID
- 39629676
- PMCID
- PMC11615840
- NLM abbreviation
- J Comp Neurol
- ISSN
- 1096-9861
- eISSN
- 1096-9861
- Publisher
- Wiley
- Grant note
- University of Iowa Department of Neurology Carver Trust & Iowa Neuroscience Institute
This work was supported in part by startup funds from the University of Iowa Department of Neurology, the University of Iowa Aging, Mind, and Brian Initiative, and an Early-Stage Investigator Award from the Carver Trust & Iowa Neuroscience Institute. We thank Carmen Birchmeier for sharing the guinea pig-anti-Lmx1b antiserum, Hideki Enomoto for sharing the guinea pig-anti-Phox2b antiserum, and Gabrielle Iverson and Mya Leuang for assistance with immunohistology. We also thank Jady Tolda for proofreading the manuscript.
- Language
- English
- Date published
- 12/01/2024
- Academic Unit
- Neurology; Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
- Record Identifier
- 9984755315802771
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