Molecular and cytological features of the mouse B-cell lymphoma line iMycEμ-1

Seong Su  Han; Arthur L Shaffer; Liangping Peng; Seung Tae Chung; Jae Hwan Lim; Sungho Maeng; Joong Su Kim; Nicole McNeil; Thomas Ried; Louis M Staudt; Siegfried Janz

doi:10.1186/1476-4598-4-40

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Molecular and cytological features of the mouse B-cell lymphoma line iMycEμ-1

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Molecular and cytological features of the mouse B-cell lymphoma line iMycEμ-1

Seong Su Han, Arthur L Shaffer, Liangping Peng, Seung Tae Chung, Jae Hwan Lim, Sungho Maeng, Joong Su Kim, Nicole McNeil, Thomas Ried, Louis M Staudt, …

Molecular cancer, Vol.4(1), pp.40-40

11/01/2005

DOI: 10.1186/1476-4598-4-40

PMCID: PMC1298327

PMID: 16277667

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Abstract

Background: Myc-induced lymphoblastic B-cell lymphoma (LBL) in iMycEmu mice may provide a model system for the study of the mechanism by which human MYC facilitates the initiation and progression of B cell and plasma cell neoplasms in human beings. We have recently shown that gene-targeted iMycEmu mice that carry a His6-tagged mouse Myc cDNA, MycHis, just 5' of the immunoglobulin heavy-chain enhancer, Emu, are prone to B cell and plasma cell tumors. The predominant tumor (approximately 50%) that arose in the iMycEmu mice on the mixed genetic background of segregating C57BL/6 and 129/SvJ alleles was LBL. The purpose of this study was to establish and characterize a cell line, designated iMycEmu-1, for the in-depth evaluation of LBL in vitro. Methods: The morphological features and the surface marker expression profile of the iMycEmu-1 cells were evaluated using cytological methods and FACS, respectively. The cytogenetic make-up of the iMycEmu-1 cells was assessed by spectral karyotyping (SKY). The expression of the inserted MycHis gene was determined using RT-PCR and qPCR. Clonotypic immunoglobulin gene arrangements were detected by Southern blotting. The global gene expression program of the iMycEmu-1 cells and the expression of 768 "pathway" genes were determined with the help of the Mouse Lymphochip(c) and Superarray(c) cDNA micro- and macroarrays, respectively. Array results were verified, in part, by RT-PCR and qPCR. Results: Consistent with their derivation from LBL, the iMycEmu-1 cells were found to be neoplastic IgMhighIgDlow lymphoblasts that expressed typical B-cell surface markers including CD40, CD54 (ICAM-1), CD80 (B7-1) and CD86 (B7-2). The iMycEmu-1 cells harbored a reciprocal T(9;11) and three non-reciprocal chromosomal translocations, over-expressed MycHis at the expense of normal Myc, and exhibited gene expression changes on Mouse Lymphochip microarrays that were consistent with MycHis-driven B-cell neoplasia. Upon comparison to normal B cells using eight different Superarray cDNA macroarrays, the iMycEmu-1 cells showed the highest number of changes on the NFkappaB array. Conclusion: The iMycEmu-1 cells may provide a uniquely useful model system to study the growth and survival requirements of Myc-driven mouse LBL in vitro.

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Title: Subtitle: Molecular and cytological features of the mouse B-cell lymphoma line iMycEμ-1
Creators: Seong Su Han - Laboratory of Genetics, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD, USA
Arthur L Shaffer - Metabolism Branch, CCR, NCI, NIH, Bethesda, MD, USA
Liangping Peng - Laboratory of Genetics, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD, USA
Seung Tae Chung - Laboratory of Genetics, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD, USA
Jae Hwan Lim - Laboratory of Metabolism, CCR, NCI, NIH, Bethesda, MD, USA
Sungho Maeng - Laboratory of Cellular Carcinogenesis and Tumor Promotion, CCR, NCI, NIH, Bethesda, MD, USA
Joong Su Kim - Laboratory of Genetics, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD, USA
Nicole McNeil - Genetics Branch, CCR, NCI, NIH, Bethesda, MD, USA
Thomas Ried - Genetics Branch, CCR, NCI, NIH, Bethesda, MD, USA
Louis M Staudt - Metabolism Branch, CCR, NCI, NIH, Bethesda, MD, USA
Siegfried Janz - Laboratory of Genetics, Center for Cancer Research (CCR), National Cancer Institute (NCI), NIH, Bethesda, MD, USA
Resource Type: Journal article
Publication Details: Molecular cancer, Vol.4(1), pp.40-40
DOI: 10.1186/1476-4598-4-40
PMID: 16277667
PMCID: PMC1298327
NLM abbreviation: Mol Cancer
ISSN: 1476-4598
eISSN: 1476-4598
Publisher: BioMed Central
Language: English
Date published: 11/01/2005
Academic Unit: Stead Family Department of Pediatrics; Pathology
Record Identifier: 9984083231302771

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