Molecular and genetic characterization of a Taf1p domain essential for yeast TFIID assembly

Madhu V Singh; Christin E Bland; P Anthony Weil

doi:10.1128/MCB.24.11.4929-4942.2004

Back

Molecular and genetic characterization of a Taf1p domain essential for yeast TFIID assembly

Journal article

Peer reviewed

Molecular and genetic characterization of a Taf1p domain essential for yeast TFIID assembly

Madhu V Singh, Christin E Bland and P Anthony Weil

Molecular and cellular biology, Vol.24(11), pp.4929-4942

06/2004

DOI: 10.1128/MCB.24.11.4929-4942.2004

PMCID: PMC416396

PMID: 15143185

Files and links (1)

url

http://doi.org/10.1128/MCB.24.11.4929-4942.2004View

Open Access

Abstract

Yeast Taf1p is an integral component of the multiprotein transcription factor TFIID. By using coimmunoprecipitation assays, coupled with a comprehensive set of deletion mutants encompassing the entire open reading frame of TAF1, we have discovered an essential role of a small portion of yeast Taf1p. This domain of Taf1p, termed region 4, consisting of amino acids 200 to 303, contributes critically to the assembly and stability of the 15-subunit TFIID holocomplex. Region 4 of Taf1p is mutationally sensitive, can assemble several Tafps into a partial TFIID complex, and interacts directly with Taf4p and Taf6p. Mutations in Taf1p-region 4 induce temperature-conditional growth of yeast cells. At the nonpermissive temperature these mutations have drastic effects on both TFIID integrity and mRNA synthesis. These data are consistent with the hypothesis that Taf1p subserves a critical scaffold function within the TFIID complex. The significance of these data with regard to TFIID structure and function is discussed.

Mutation

TATA-Binding Protein Associated Factors - metabolism

Transcription Factor TFIID - chemistry

Temperature

Saccharomyces cerevisiae - genetics

Protein Structure, Tertiary - genetics

Saccharomyces cerevisiae Proteins - genetics

Transcription Factor TFIID - metabolism

TATA-Binding Protein Associated Factors - genetics

Saccharomyces cerevisiae - metabolism

Transcription Factor TFIID - genetics

Transcription Factor TFIID - biosynthesis

Saccharomyces cerevisiae Proteins - metabolism

Protein Structure, Tertiary - physiology

Details

Title: Subtitle: Molecular and genetic characterization of a Taf1p domain essential for yeast TFIID assembly
Creators: Madhu V Singh - Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, Nashville, TN 37232-0615, USA
Christin E Bland
P Anthony Weil
Resource Type: Journal article
Publication Details: Molecular and cellular biology, Vol.24(11), pp.4929-4942
DOI: 10.1128/MCB.24.11.4929-4942.2004
PMID: 15143185
PMCID: PMC416396
NLM abbreviation: Mol Cell Biol
ISSN: 0270-7306
eISSN: 1098-5549
Grant note: R01 GM052461 / NIGMS NIH HHS R56 GM052461 / NIGMS NIH HHS GM52461 / NIGMS NIH HHS
Language: English
Date published: 06/2004
Academic Unit: Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984094392402771

Metrics

11 Record Views

14 Times Cited - Web of Science