Journal article
Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization and infection isolates in a Veterans Affairs hospital
Antimicrobial resistance and infection control, Vol.4(1), pp.10-10
2015
DOI: 10.1186/s13756-015-0048-5
PMCID: PMC4383227
PMID: 25838886
Abstract
Nasal colonization with methicillin-resistant Staphylococcus aureus (MRSA) is associated with increased infection risk, yet colonization and infection isolates are rarely compared within the same study. The objectives of this study were to compare colonization and infection isolates from a Veterans Administration hospital in Iowa, and to determine the prevalence of livestock-associated MRSA (LA-MRSA) colonization and infection in a state with high livestock density. All patients with available MRSA isolates collected through routine nasal screening (73%; n = 397) and from infections (27%; n = 148) between December 2010 and August 2012 were included and tested for spa type and presence of PVL and mecA genes. Clinical isolates were tested for antibiotic resistance patterns. Paired colonization and infection isolates were compared for genetic and phenotypic congruity. The most common spa types were t002 (and other CC5-associated strains; 65%) and t008 (and other CC8-associated strains; 20%). No classic LA-MRSA spa types were identified. CC5-associated strains were less likely to be associated with infections (22%; 77/353) compared with CC8-associated strains (49%; 53/109). MRSA colonization was more common among patients with infections (71%) compared with the general screening population (7%). In most cases (82%; 28/34), paired colonization and infection isolates were genetically and phenotypically indistinguishable. Our data demonstrate a direct link between antecedent nasal colonization and subsequent MRSA infection. Further, our data indicate variability in colonization and infection efficiency among MRSA genotypes, which points to the need to define the molecular determinants underlying emergence of S. aureus strains in the community and nosocomial setting.
Details
- Title: Subtitle
- Molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization and infection isolates in a Veterans Affairs hospital
- Creators
- Kalyani E Eko - Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA 52246 USABrett M Forshey - Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA 52246 USAMargaret Carrel - Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA 52246 USA ; Department of Geographical & Sustainability Sciences, University of Iowa, Iowa City, IA 52242 USAMarin L Schweizer - Center for Comprehensive Access & Delivery Research and Evaluation (CADRE), Iowa City VA Health Care System, Iowa City, IA 52246 USA ; Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52246 USAEli N Perencevich - Center for Comprehensive Access & Delivery Research and Evaluation (CADRE), Iowa City VA Health Care System, Iowa City, IA 52246 USA ; Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA 52246 USATara C Smith - Department of Epidemiology, University of Iowa College of Public Health, Iowa City, IA 52246 USA ; Department of Biostatistics, Environmental Health Sciences and Epidemiology, College of Public Health, Kent State University, Kent, OH 44242 USA
- Resource Type
- Journal article
- Publication Details
- Antimicrobial resistance and infection control, Vol.4(1), pp.10-10
- DOI
- 10.1186/s13756-015-0048-5
- PMID
- 25838886
- PMCID
- PMC4383227
- NLM abbreviation
- Antimicrob Resist Infect Control
- ISSN
- 2047-2994
- eISSN
- 2047-2994
- Publisher
- England
- Grant note
- K01 OH009793 / NIOSH CDC HHS
- Language
- English
- Date published
- 2015
- Academic Unit
- Epidemiology; Interdisciplinary Programs; Geographical and Sustainability Sciences; Internal Medicine
- Record Identifier
- 9983779492002771
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