Journal article
Molecular characterization of suppression of hepatitis B virus transcription by hepatitis C virus core protein
Science in China. Series C, Life sciences, Vol.40(6), pp.648-656
12/01/1997
DOI: 10.1007/BF02882696
PMID: 18726290
Abstract
To further elucidate the molecular mechanisms underlying the suppression of hepatitis B virus (HBV) expression by the hepatitis C virus (HCV) core protein, five molecular clones of HCV cDNA sequence containing the 5' noncoding (5'NC) and the core regions have been isolated from Chinese HBV- and HCV-coinfected patients. Sequence comparison and phylogenetic analysis showed that the HCV sequence cloned from coinfected individuals is indistinguishable from that identified in other patients. Cotransfection assay confirmed that the core protein expressed from one of the cloned sequence is capable of suppressing the expression of hepatitis B surface and e antigens (HBsAg and HBeAg, respectively). Deletion mapping revealed that the Cterminal hydrophobic region of the HCV. core is necessary for the suppression. Results from reporter assays demonstrated that HCV core protein interacts with the HBV C promoter and enhancer II elements and down-regulates the transcription of HBV as well as other cellular and heterologous viral genes in both hepatic and non-hepatic cell lines. Taken together, the findings suggest HCV core protein as a multifunctional negative regulator of transcription critically involved in the molecular interactions between HBV and HCV, and between HCV and the cell.
Details
- Title: Subtitle
- Molecular characterization of suppression of hepatitis B virus transcription by hepatitis C virus core protein
- Creators
- Hailin Wang - Institute of VirologyZiying YanYunde HouDongyan Jin
- Resource Type
- Journal article
- Publication Details
- Science in China. Series C, Life sciences, Vol.40(6), pp.648-656
- DOI
- 10.1007/BF02882696
- PMID
- 18726290
- ISSN
- 1006-9305
- eISSN
- 1862-2798
- Language
- English
- Date published
- 12/01/1997
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284343702771
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