Journal article
Molecular cloning and characterization of the purE operon of Escherichia coli
Gene, Vol.44(1), pp.55-62
1986
DOI: 10.1016/0378-1119(86)90042-9
PMID: 3021590
Abstract
The
purE operon of
Escherichia coli has been cloned and localized to a 1.7-kb
HpaI fragment. The operon has been further characterized by subcloning into the
lac fusion vector, pMC1403, and by the construction of BAL 31-generated deletions. The
purE regulation region has been identified by assay of β-galactosidase produced by
pur-lac fusion plasmids and by RNA polymerase binding to end-labelled restriction fragments. Two
purE promoters have been identified; one strong that is regulated by purines, the other weaker which is not regulated. The latter may be internal to the
purE
1 structural gene.
Details
- Title: Subtitle
- Molecular cloning and characterization of the purE operon of Escherichia coli
- Creators
- John KamholzJacqueline KeyhaniJoseph S Gots
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.44(1), pp.55-62
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0378-1119(86)90042-9
- PMID
- 3021590
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 1986
- Academic Unit
- Neurology; Psychiatry
- Record Identifier
- 9984020883802771
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