Molecular cloning of Ian4: a BCR/ABL-induced gene that encodes an outer membrane mitochondrial protein with GTP-binding activity

L Dahéron; T Zenz; L D Siracusa; C Brenner; B Calabretta

doi:10.1093/nar/29.6.1308

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Molecular cloning of Ian4: a BCR/ABL-induced gene that encodes an outer membrane mitochondrial protein with GTP-binding activity

Journal article

Open access

Peer reviewed

Molecular cloning of Ian4: a BCR/ABL-induced gene that encodes an outer membrane mitochondrial protein with GTP-binding activity

L Dahéron, T Zenz, L D Siracusa, C Brenner and B Calabretta

Nucleic acids research, Vol.29(6), pp.1308-1316

03/15/2001

DOI: 10.1093/nar/29.6.1308

PMCID: PMC29751

PMID: 11238997

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Abstract

Using the representation difference analysis technique, we have identified a novel gene, Ian4, which is preferentially expressed in hematopoietic precursor 32D cells transfected with wild-type versus mutant forms of the Bcr/Abl oncogene. Ian4 expression was undetectable in 32D cells transfected with v-src, oncogenic Ha-ras or v-Abl. Murine Ian4 maps to chromosome 6, 25 cM from the centromere. The Ian4 mRNA contains two open reading frames (ORFs) separated by 5 nt. The first ORF has the potential to encode for a polypeptide of 67 amino acids without apparent homology to known proteins. The second ORF encodes a protein of 301 amino acids with a GTP/ATP-binding site in the N-terminus and a hydrophobic domain in the extreme C-terminus. The IAN-4 protein resides in the mitochondrial outer membrane and the last 20 amino acids are necessary for this localization. The IAN-4 protein has GTP-binding activity and shares sequence homology with a novel family of putative GTP-binding proteins: the immuno-associated nucleotide (IAN) family.

Haplotypes

Humans

DNA, Complementary - genetics

Molecular Sequence Data

Male

GTP-Binding Proteins - genetics

RNA - genetics

Cloning, Molecular

Female

Membrane Proteins - metabolism

Tumor Cells, Cultured

RNA - metabolism

Amino Acid Sequence

Cell Line

Membrane Proteins - genetics

Gene Expression Regulation

Chromosome Mapping

Mitochondria - metabolism

Sequence Analysis, DNA

Sequence Homology, Amino Acid

Microscopy, Confocal

Fusion Proteins, bcr-abl - genetics

Sequence Alignment

Animals

Fusion Proteins, bcr-abl - physiology

K562 Cells

Mice

DNA, Complementary - chemistry

Intracellular Membranes - metabolism

Crosses, Genetic

GTP-Binding Proteins - metabolism

Details

Title: Subtitle: Molecular cloning of Ian4: a BCR/ABL-induced gene that encodes an outer membrane mitochondrial protein with GTP-binding activity
Creators: L Dahéron - Thomas Jefferson University, Department of Microbiology and Immunology, Kimmel Cancer Institute, Philadelphia, PA 19107, USA
T Zenz
L D Siracusa
C Brenner
B Calabretta
Resource Type: Journal article
Publication Details: Nucleic acids research, Vol.29(6), pp.1308-1316
DOI: 10.1093/nar/29.6.1308
PMID: 11238997
PMCID: PMC29751
NLM abbreviation: Nucleic Acids Res
ISSN: 0305-1048
eISSN: 1362-4962
Publisher: England
Grant note: CA21124 / NCI NIH HHS
Language: English
Date published: 03/15/2001
Academic Unit: Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9983788430602771

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